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Thermus thermophilus UTM802 and application thereof

A technology of thermophilic thermophilic bacteria and strains, applied in the field of environmental microorganisms, can solve the problems of reducing the efficacy and inactivation of inoculants, and achieve the effects of low cost, avoiding inactivation, and rapid degradation

Active Publication Date: 2013-08-21
北京绿源科创环境技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The active ingredients in these microbial inoculants are often inhibited or even inactivated in the compost high temperature environment of 50°C-80°C, which greatly reduces the efficacy of the inoculant
However, there has been no report on the application of Thermus thermophilus as an inoculant in high-temperature composting

Method used

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  • Thermus thermophilus UTM802 and application thereof
  • Thermus thermophilus UTM802 and application thereof
  • Thermus thermophilus UTM802 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The screening of embodiment 1 thermophilic Thermus UTM802

[0034] Weigh 5g of high-temperature period sludge compost samples into a 150mL Erlenmeyer flask filled with 15 glass beads and 50mL of sterile water, place on a shaker and vibrate at 160rpm for 1 hour to fully disperse the samples, then place them at 70°C and let them stand 3 hours.

[0035] Take a sterile petri dish, absorb 1mL high-temperature enrichment solution and low-fold dilution solution, and insert it into the marked petri dish; pour the improved YTPG medium that has been melted and cooled to about 55°C into a sterile petri dish. The culture dish is about 30mL, gently shake the culture dish back and forth, mix well with the enrichment solution, and wait for solidification.

[0036] Put the solidified plates upside down in airtight containers, and at the same time, place a petri dish filled with distilled water in each airtight container, and place them in an incubator for cultivation at 70°C. Observe...

Embodiment 2

[0038] Example 2 Identification of Thermus thermophilus UTM802

[0039] Thermophilus Thermus UTM802 obtained in Example 1 was cultured on the improved YTPG medium plate at 70°C for 24 hours, and its characteristics were observed. As a result, it was a Gram-negative, aerobic bacterium and did not form spores. It is short rod-shaped.

[0040] Using the DNA of the strain UTM802 of the present invention as a template, its 16S rRNA sequence was amplified by PCR, and the primers were (27f): 5′-AGA GTT TGA TCC TGG CTC AG-3′ and (1492r): 5′-GGT TAC CTT GTTACG ACT T-3'. The PCR reaction program was: pre-denaturation at 95°C for 5 min, denaturation at 95°C for 30 s, annealing at 53°C for 45 s, extension at 72°C for 90 s, 30 cycles, and extension at 72°C for 10 min. The purity of the amplified product was checked by electrophoresis and then sequenced. The sequencing result is shown in SEQ ID NO.1 in the sequence table. The obtained 16S rDNA gene sequence was compared with the EzTaxon ...

Embodiment 3

[0043] The temperature range of the growth of embodiment 3 thermophilic Thermus UTM802

[0044] The UTM 802 strain was inoculated on the modified YTPG medium plate and activated at 70°C for 24 hours. Then it was inoculated on the improved YTPG medium, and cultured at the culture temperature of 40°C, 50°C, 60°C, 70°C, and 80°C.

[0045] According to the temperature mark, put them into different incubators for cultivation, observe the growth of the strain after 24 hours, and identify the growth of the strain. Fill in the observations in Table 1 below. "-" means no growth; "+" means slight growth; "++" means moderate growth; "+++" means good growth.

[0046] Table 1 Effects of different temperatures on microbial growth

[0047] Culture temperature (℃)

[0048] The results showed that the strain UTM 802 could grow at 50°C~80°C, and it grew well at 60°C and 70°C, which indicated that the strain UTM802 was thermophilic and belonged to thermophilic thermophilic bacteria....

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Abstract

The invention relates to thermus thermophilus UTM802 preserved with CGMCC (China General Microbiological Culture Collection Center) No.6186. In a bacterial strain of the thermus thermophilus, organic waste is utilized as a carrier, solid-state high-temperature microbe compost bactericide is prepared in low cost and has no effect on moisture of materials during compost. In high-temperature compostby the solid-state high-temperature microbe compost bactericide, inactivation of active microbes in inoculant during high-temperature fermenting can be avoided, and the thermus thermophilus UTM802 has the advantages of stable performance and convenience in use.

Description

technical field [0001] The present invention relates to the field of environmental microorganisms, in particular to the thermophilic Thermus UTM802 and its application. Background technique [0002] Thermus thermophilus (Thermus thermophilus) is a kind of aerobic, Gram-negative, non-bacillus bacteria that cannot grow below 40°C, the optimum growth temperature is 65-70°C, and the maximum growth temperature is 80°C. cell wall structure. Thermus is a typical representative of high temperature bacteria, and it is a key group of bacteria in basic research and applied research of high temperature biology. It is closely related to hydrothermal environment and widely exists in geothermal hot springs, submarine craters and artificial hydrothermal environments pipes and high-temperature aerobic composting). [0003] Traditional composting generally uses indigenous microorganisms in composting materials, which often have problems such as long fermentation time, serious odor pollution...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/00C12R1/01
CPCY02W30/40
Inventor 刘永跃周顺桂许宜北汪涌
Owner 北京绿源科创环境技术有限公司
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