Longan functional drink and method for preparing same
A technology of functional drinks and longan, applied in the food field, can solve the problems of not fully utilizing the nutritional content of longan and lack of nutritional improvement
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Embodiment 1
[0033] Example 1: Cloning of the manganese superoxide dismutase gene and expression in Lactobacillus bulgaricus
[0034] (1) Preparation of Bulgarian lactic acid bacteria containing manganese superoxide dismutase gene
[0035] Amplification primers were designed according to the nucleotide sequence of Lactococcus superoxide dismutase gene (SOD) in GenBank (Genbank sequence number: NC_002662):
[0036] P1: 5'-CGCGGATCCGCGATGGCATTTACATTA-3'; (SEQ ID NO: 1)
[0037] P2: 5'-CCGCTCGAG CGGTTATTTTGCTTTAGCAT-3'. (SEQ ID NO:2)
[0038] PCR amplification program (95°C, 5min; 95°C, 1min; 55°C, 1min; 72°C, 1min; 30 cycles; 72°C, 7min), the template was derived from Lactococcus lactis (purchased from Guangdong Institute of Microbiology species collection) genomic DNA. The carrier plasmid is food-grade PMG36e (purchased from Shanghai Beinuo Biotechnology Co., Ltd.);
[0039] (2) Lactobacillus delbrueckii subsp. bulgaricus was transformed by electroporation with the recombinant plasmid ...
Embodiment 2
[0041] Mix 5 kg of longan meat, 0.3 kg of red dates, and 0.1 kg of wolfberry, add water to 50 L, boil to 90 ° C and keep warm for 30 minutes, grind and filter with a homogenizer to obtain longan red dates and wolfberry juice, and inoculate 2% of the manganese superoxide obtained in Example 1 Lactobacillus bulgaricus with the dismutase gene is fermented in a closed anaerobic manner at 40°C for 3 days, and after the fermentation is completed, it is rapidly cooled to below 10°C for storage.
Embodiment 3
[0043] Mix 5 kg of longan meat, 0.4 kg of red dates, and 0.02 kg of rosemary, add water to 50 L, boil to 80°C and keep warm for 40 minutes, grind and filter with a homogenizer to obtain longan and red date juice, and inoculate 3% of the manganese superoxide obtained in Example 1. The Lactobacillus bulgaricus with the dismutase gene was fermented in a closed anaerobic manner at 37°C for 4 days. After the fermentation, it was quickly cooled to below 10°C for storage.
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