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Trichoderma asperellum strain

A technology of Trichoderma aculeatus and Trichoderma, applied in the field of microorganisms, can solve the problems of failure to prevent gray mold, damage to the grape growing environment, and large dosage, so as to reduce the occurrence of grape gray mold and inhibit grape ash The effect of mildew

Active Publication Date: 2013-04-17
SHANGHAI WANLIHUA BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the process of using pesticides in the treatment of botrytis cinerea, there is often a problem of large dosage. If the dosage is small, the effect of preventing and treating botrytis will not be achieved. Long-term large-scale use of pesticides will damage the grape growing environment.
Although a good effect of preventing Botrytis cinerea can be achieved in a short period of time, the use of pesticides as a method of preventing Botrytis cinerea will be eliminated in the long run

Method used

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  • Trichoderma asperellum strain
  • Trichoderma asperellum strain
  • Trichoderma asperellum strain

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Embodiment Construction

[0027] The present invention will be explained in more detail with the help of the following examples. The following examples are only illustrative, and it should be understood that the present invention is not limited by these examples.

[0028] Strain screening method

[0029] Weigh 1g soil sample in 10ml water, mix it on a vortexer, and dilute it to 10 -5 , Take 1ml and add it to the surface of the Trichoderma selective medium TSM medium, spread it evenly, and let the medium stand for 5-7 days at 25°C. According to the morphology, color and size of the fungal colonies screened on the plate, the Trichoderma colonies were selected and transferred to the PDA medium for purification, culture and further identification.

[0030] TSM medium formula (unit: g / l): MgSO 4 7H 2 O 0.2g, KH 2 PO 4 0.9g, KCl 0.15g, NH 4 NO 3 1.0g, glucose 3g, chloramphenicol 0.25g, methanesulfonate 0.3g, pentachloronitrobenzene 0.2g, rose bengal 0.15g, agar 20g.

[0031] Strain culture

[0032] Add 100 mL of...

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Abstract

A Trichoderma asperellum strain has an accession number of CGMCC No. 5201.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a Trichoderma strain with high resistance to botrytis cinerea. Background technique [0002] Botrytis cinerea mainly damages inflorescences, young fruits and fruits that are about to mature, and can also infect fruit stems, new shoots and young leaves. Botrytis cinerea is caused by Deuteromycotina, Hyphospora, Hyphospora, Botrytis Botrytis cinerea pers.ex Fr. caused by infection. The pathogen is a facultative parasitic fungus with a wide range of hosts. It can parasitize a variety of fruits, vegetables and flowers, so the pathogen has a wide range of infecting agents. [0003] For the disease, the conventional method is to use spraying insecticides to prevent and control botrytis cinerea. In Chinese patent CN101543217, it is disclosed that a fungicidal composition containing cyprodinil and tebuconazole is used to prevent and treat botrytis cinerea, and Chinese patent CN101731...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 张钰王承芳旷文丰干华磊陈娟毛伟力
Owner SHANGHAI WANLIHUA BIOTECH
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