Method for separating anti-rotavirus immunoglobulin from chicken egg

A technology of immunoglobulin and rotavirus, applied in the direction of antiviral immunoglobulin and immunoglobulin from eggs, etc., to achieve the effect of high separation efficiency, quality assurance and simple process

Active Publication Date: 2014-11-05
孙亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the technical problem of separation and extraction of anti-rotavirus immunoglobulin in chicken eggs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] 1. Constant temperature transformation of chicken eggs: After the chicken eggs are separated from the mother, they are collected in a container, and the transformation is carried out at a temperature of 18-25°C for 120 hours. Proliferate antibody immunoglobulin molecules in chicken eggs.

[0012] 2. Dilute eggs with water:

[0013] Broken chicken eggs that have been converted at a constant temperature, took out the yolk and egg white, and added one-fold mass of egg yolk and egg white to five-fold mass of deionized water to form chicken egg liquid.

[0014] 2. The first settling: inject the chicken egg liquid into the container, settle at room temperature for 24 hours, initially separate the egg yolk precipitate and egg white liquid, pour the egg white liquid to separate, collect the egg white liquid, and remove the egg yolk precipitate .

[0015] 3. The second settling: continue to settle the egg white liquid after the first settling at room temperature for 48 hours. ...

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PUM

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Abstract

The invention provides a method for separating anti-rotavirus immunoglobulin from a chicken egg. The method comprises the process steps of: transforming the chicken egg at the constant temperature, breaking the egg, adding water for dilution, performing first sedimentation and performing second sedimentation. The adoption of the method disclosed by the invention for separating the anti-rotavirus immunoglobulin from the chicken egg inoculated with rotavirus has the characteristics of simple process, few equipment, low construction cost, capability of ensuring quality and high separation efficiency. The method is suitable for separating the anti-rotavirus immunoglobulin from the chicken egg after the chicken egg is inoculated with the rotavirus.

Description

technical field [0001] The invention proposes a method for extracting substances in the field of biochemistry, specifically a method for separating anti-rotavirus immunoglobulins in chicken eggs. Background technique [0002] After rotavirus infects the human body, it will cause damage to the digestive system of children, causing seasonal diarrhea, and even affecting health or causing death from dehydration. In order to prepare anti-rotavirus antibodies, the usual method is: inject the rotavirus into the body of the chicken, after a period of time, the chicken will produce antibodies to the rotavirus, and the antibodies will accumulate in various organs in the body of the chicken, and also accumulate In chicken eggs, in order to obtain anti-rotavirus immunoglobulin, it needs to be extracted from chicken eggs. Before the present invention proposes, anti-rotavirus immunoglobulin is separated by gel chromatographic column or separated by centrifuge, wherein, the separation pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C07K16/02C07K1/30
Inventor 孙亮
Owner 孙亮
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