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Method for collecting bolete spores

A collection method and technology for boletus, which are applied in the fields of botanical equipment and methods, plant preservation, agricultural machinery and implements, etc., can solve the problems of artificial cultivation, etc., and achieve the effects of easy operation, pure spores, and large collection amount.

Inactive Publication Date: 2013-05-22
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Boletus stipeii can not be artificially cultivated

Method used

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  • Method for collecting bolete spores
  • Method for collecting bolete spores
  • Method for collecting bolete spores

Examples

Experimental program
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Embodiment

[0061] The present invention will be further described below through examples, but these examples are for illustration purposes only and should not be construed as limiting the scope of the present invention.

[0062] Preparation of boletus spore collection kit

[0063] Plastic normal temperature group: Take 250ml wide-mouth glass bottles and several caps one day before collection, lay a piece of round filter paper on the bottom of the bottle, and seal the cap; prepare several sheets of 20cm X 20cm long and wide kraft paper or newspaper, several rubber bands, 5-10 bacterial blades, scissors and tweezers. After the above items were autoclaved at 121°C for 30 minutes, they were placed in a plastic normal temperature box sterilized with alcohol surface for standby. Incubator group: On the basis of collection tool 1, prepare 1-2 incubators for surface alcohol disinfection. All utensils are commercially available products, without special products of the present invention.

[00...

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Abstract

The invention provides a method for collecting bolete spores. The method comprises the following steps: 1) sporocarp collecting step, a pileus which does not diffuse spores is placed on an inlet of a collecting container in a mode that bolete holes face downwards, wherein filter paper is inlaid on the bottom portion of the collecting container, and the pileus and the collecting container are wrapped together by using aseptic wrapping materials; 2) low temperature storage step, the collecting container is stored in a heat preservation box with temperature from 2 DEG C to 15 DEG C; and 3) spore obtaining step, the pileus is placed in the temperature condition from 2 DEG C to 5 DEG C so as to enable the spores to be released on a cushion layer. The invention further provides a method for storing the bolete spores. The collecting method and the storing method have the advantages of being simple, easy to operate, large in collection amount, pure in spores and the like.

Description

technical field [0001] The invention relates to a method for collecting and preserving mycorrhizal edible fungus strains, in particular to a method for collecting and preserving boletus spores. Background technique [0002] Boletus belongs to the family Boletaceae (Boletaceae) in the Basidiomycotina Agaricaceae. Except for a few varieties that are poisonous or bitter and cannot be eaten, most of the varieties are edible. There are mainly white, yellow and black boletus. White boletus is delicious and nutritious. The fungus has a large body, thick flesh, strong stalk, sweet and delicious taste, and rich nutrition. It is a world-renowned edible fungus. People of all ethnic groups in Yunnan Province like to collect fresh mushrooms for cooking. Western European countries also have the habit of eating white boletus widely. In addition to fresh cooking, most of them are sliced ​​and dried, processed into various small packages, used to prepare soup or made into soy sauce extrac...

Claims

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Application Information

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IPC IPC(8): A01D91/04A01N3/00
Inventor 陈青君牛玉蓉张国庆程继鸿王明花杨瑞
Owner BEIJING UNIV OF AGRI
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