Method for determining thermo sensitive genetic male sterile line through sowing at different stages in cold water treatment period

Abstract

The invention discloses a method for determining a thermo sensitive genetic male sterile line through sowing at different stages in a cold water treatment period. Seeds of a sterile line to be treated with cold water are divided into two parts, A and B, wherein the part A is sown on a certain day during April 20th and May 10th and served as an early sterile line, and the part B is sown 9-11 days after the part A is sown and used as a late sterile line; the parts A and B are sown and transplanted under normal field management, the plant morphological change of the part A is closely observed during the end of June and the beginning of July, and the part B is immediately and correspondingly barreled and put into a cold water treatment tank to be treated for 6-8 days and then is taken out during a flat pulvinus period of the part A; and the last and penultimate tassels which have a pulvinus distance of+ / -1cm are selected, and the residual tassels are removed by scissors, so that the thermo sensitive genetic male sterile line is obtained. The method has the advantages of simplicity, efficiency, low cost, strong environmental controllability and convenience in operation.

Description

technical field [0001] The invention relates to the field of a new treatment method for utilizing new materials for rice heterosis, and specifically relates to a method for determining the cold water treatment period of a temperature-sensitive genital male sterile line by staged sowing. Background technique [0002] Two-line hybrid rice varieties, which combine excellent traits such as high yield, high quality and strong resistance, have been widely promoted. The two-line hybrid rice is based on the thermosensitive genic male sterile material as the female parent to prepare the hybrid rice combination to make use of heterosis for rice. The male sterility of the thermosensitive genic male sterile line of rice can produce hybrids under long-day high temperature conditions, and short-day low temperature conditions can produce hybrids. Males are fertile enough to reproduce themselves. The expression of sterility in thermosensitive genic male sterile lines depends on the inducti...

AI Technical Summary

Problems solved by technology

The expression of sterility in thermosensitive genic male sterile lines depends on the induction of long-day high temperature in the external environment during the thermosensitive sterility sensitive period, that is, the pollen mother cell formation stage to the pollen mononucleate stage in rice panicle differentiation. However, in midsummer The temperature-sensitive male sterile line of rice in the sterility-sensitive period encounters an unexpected relatively low temperature, and there is a huge risk that the sterility cannot be fully induced, resulting in the failure of hybrid seed production. Reducing or avoiding this production risk is the successful promotion of two-line rice. The essential

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