Gene-detection integrally completed enclosed PCR (polymerase chain reaction) amplification tube

A genetic detection, closed technology, applied in the direction of specific-purpose bioreactors/fermenters, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of reducing the reliability of experimental detection, increasing pollution, etc., to improve reliability performance and accuracy, and the effect of avoiding contamination

Inactive Publication Date: 2014-07-23
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the gene chip can be used to obtain high-throughput gene information and detect multiple genes at the same time, the amplification of the gene to be tested, the transfer of the amplified product, and the hybridization are all completed in different systems. It will inevitably increase the possibility of contamination, thereby reducing the reliability of experimental detection

Method used

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  • Gene-detection integrally completed enclosed PCR (polymerase chain reaction) amplification tube
  • Gene-detection integrally completed enclosed PCR (polymerase chain reaction) amplification tube
  • Gene-detection integrally completed enclosed PCR (polymerase chain reaction) amplification tube

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Embodiment 1

[0019] The closed PCR amplification tube for integrated gene detection of the present invention includes a tube body 1 for containing PCR buffer, a liquid storage pool 2 for containing hybridization buffer, and a tube cover that seals the upper opening of the liquid storage pool 2 3. The liquid storage tank 2 is put into the upper part of the tube body 1, the upper end of the liquid storage tank 2 is airtightly connected with the opening upper end of the tube body 1, and the bottom surface of the liquid storage tank 2 is provided with a connecting pipe 21 connecting the upper and lower sides of the bottom surface. 21 The lower end is connected with the bottom surface. The reservoir 2 and the cap 3 are assembled together by ultrasonic fusion, and are used to store the complete hybridization system. Wherein, the liquid storage tank 2 adopts a dark color, and two protrusions 22 are arranged on the outer edge of the bottom of the liquid storage tank 2, so that the liquid storage t...

Embodiment 2

[0030] The closed PCR amplification tube with integrated gene detection of the present invention includes a freely assembling cap and a tube body, and is used for detecting transgenic soybeans. The cap 3 is fixed with a specific probe for transgenic soybean transfer gene through a spotting instrument, and the liquid reservoir 2 and the tube body 1 are used to store the hybridization system and the amplification system respectively. Design specific primers and add them to the tube body 1 together with the gene to be tested. After amplification, invert the amplification tube so that the amplified product enters the tube cap. needle hybridization reaction. Fluorescence scanning, if there is fluorescence, the gene is amplified, the gene to be tested contains the transferred gene, and the sample comes from transgenic soybean.

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Abstract

The invention discloses a closed PCR amplification tube for integrated gene detection, which includes a tube body for containing PCR buffer, a liquid storage pool for containing hybridization buffer, and a cap for sealing the upper opening of the liquid storage pool. The liquid storage tank is put into the upper part of the pipe body, and the upper end of the liquid storage tank is airtightly connected with the opening upper end of the pipe body. The invention not only enables PCR, gene hybridization, and gene detection to be completed in a closed system, avoiding cross-contamination, but also completely isolates the PCR amplification system and DNA hybridization system, and avoids components such as buffers and enzymes in the amplification system The impact on the hybridization reaction greatly improves the accuracy and repeatability of genetic testing. The tube cap in the closed PCR amplification tube of the present invention makes adding samples easier, has stronger sealing performance, and has better detection accuracy.

Description

technical field [0001] The invention relates to a closed PCR amplification tube, which can be used for integrated detection of gene information, that is, corresponding functions such as gene amplification, hybridization, and detection are sequentially completed in the same closed space. Background technique [0002] At present, polymerase chain reaction is one of the indispensable technologies in various detection and research processes involving nucleic acid molecules, such as molecular biology, gene detection, disease detection and diagnosis. As we all know, PCR (Polymerase Chain Reaction, Polymerase Chain Reaction), the amplification method is extremely sensitive, and can amplify the gene to be tested at a low concentration to tens of thousands to millions of times. It can be detected by electrophoresis. Because of the high sensitivity of PCR amplification, cross-contamination is prone to occur during the amplification process, and the contamination is easily amplified, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/24
Inventor 刘全俊叶晓峰于静静侯传荣吴宏文刘丽萍刘航
Owner SOUTHEAST UNIV
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