Point mutation gene detection method and kit thereof

Abstract

The invention discloses a point mutation gene detection method. The method comprises the following steps: forming a complete oligonucleotide chain by treating a mutant gene as a template and connecting two oligonucleotide probes complementary and adjacent to the point mutation gene through a connection reaction, and marking the oligonucleotide chain with electrochemical luminescence molecules; and capturing through magnetic microspheres to separate out the complete oligonucleotide chain, detecting the electrochemical luminescence molecules marked on the complete oligonucleotide chain through using an electrochemical luminescence analyzer, and comparing the electrochemical luminescence signal intensities of a gene sample to be tested and a blank solution to find the difference in order to detect the point mutation gene. The invention also discloses a pint mutation gene detection kit. The kit includes two oligonucleotide probes complementary to the point mutation gene, a ligase, and magnetic microspheres having surfaces marked with the capturing molecules. The point mutation gene detection method has the advantages of high detection sensitivity, and accurate and reliable detection result, and has a very good application prospect in the tumor molecule diagnosis field, the genetic molecule diagnosis field and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method and a kit for detecting point mutation genes. Background technique [0002] With the development of molecular biology, more and more evidences show that major diseases such as malignant tumors and genetic diseases are closely related to gene mutations, and point mutations are the most common types of gene mutations. Mutation gene detection technology has been developed rapidly. At present, gene sequencing technology is the "gold standard" for detecting gene mutations. However, due to the interference of wild-type gene background when detecting mutant genes, sequencing technology is limited by sensitivity, and it is difficult to detect mutant genes with an abundance of less than 10%. Also due to the interference of wild-type genes, polymerase chain reaction (PCR) cannot amplify and enrich low-abundance mutant genes, and it is difficult to increase the proportion ...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the technical problems that the current point mutation detection sensitivity is not high, and the detection process is easily interfered by wild-type genes in samples, and provides a method for detecting point mutation genes, which avoids the interference of wild-type genes and improves the efficiency of point mutation genes. Sensitivity of detection

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