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RNAi-mediated inhibition of tumor necrosis factor alpha-related conditions

A condition, the technology of the subject, applied in the direction of DNA / RNA fragments, recombinant DNA technology, medical preparations containing active ingredients, etc., can solve the problem of not teaching the target sequence, etc.

Inactive Publication Date: 2013-09-11
ARROWHEAD RES CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the publication does not teach specific target sequences for RNA inhibition as provided herein

Method used

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  • RNAi-mediated inhibition of tumor necrosis factor alpha-related conditions
  • RNAi-mediated inhibition of tumor necrosis factor alpha-related conditions
  • RNAi-mediated inhibition of tumor necrosis factor alpha-related conditions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0152] Example 1 Interfering RNA for specifically silencing TNFR1 in GTM-3 cells

[0153] This study examined the ability of TNFR1 interfering RNA to knock down endogenous TNFR1 protein expression levels in cultured GTM-3 cells.

[0154] Using TNFR1 siRNAs, siCONTROL RISC-free-siRNA #1, or siCONTROL Non-Targeting siRNA #2 (NTC2) and DHARMAFECT Standard in vitro concentrations (0.1-10 nM) of #1 transfection reagent (Dharmacon, Lafayette, CO) accomplished transfection of GTM-3 cells (Pang, I.H. et al., 1994. Curr. Eye Res. 13:51-63). Dissolve all siRNA in 1X siRNA buffer, which is 20mM KCl, 6mM HEPES (pH 7.5), 0.2mM MgCl 2 of aqueous solution. Control samples included a buffer control in which the volume of siRNA was replaced with an equal volume of IX siRNA buffer (-siRNA). Western blotting was performed using an anti-TNFR antibody (Santa Cruz Biotechnology, Santa Cruz, CA) to assess TNFRl protein expression. TNFR1 siRNA is a double-stranded interfering RNA specific for th...

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Abstract

RNA interference is provided for inhibition of tumor necrosis factor alpha (TNFalpha) by silencing TNFalpha cell surface receptor TNF receptor-1 (TNFR1) mRNA expression, or by silencing TNFalpha converting enzyme (TACE / ADAM17) mRNA expression. Silencing such TNFalpha targets, in particular, is useful for treating patients having a TNFalpha-related condition or at risk of developing a TNFalpha-related condition such as the ocular conditions dry eye, allergic conjunctivitis, or ocular inflammation, or such as dermatitis, rhinitis, or asthma, for example.

Description

[0001] This application is a divisional application of the Chinese patent application 200780017777.7 (International Application No. PCT / US2007 / 069165) with the filing date of May 17, 2007, and the title of the invention is "RNAi-mediated inhibition of tumor necrosis factor alpha-related conditions". [0002] related application [0003] This application claims priority to co-pending U.S. Provisional Patent Application Serial No. 60 / 801,788, entitled "RNAi-Mediated Inhibition of Tumor Necrosis Factor Alpha-Associated Conditions," filed May 19, 2006, the contents of which are expressly incorporated This article is for reference. field of invention [0004] The application relates to the field of interference RNA composition, which is used to silence tumor necrosis factor α (TNFα) by silencing TNFα cell surface receptor TNF receptor-1 (TNFR1) mRNA or TNFα converting enzyme (TACE / ADAM17) mRNA . Silencing such TNFα targets is useful in the treatment of patients suffering from or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/713A61P29/00A61P27/02A61P17/04A61P7/10A61P11/06A61P11/02C12N15/113
CPCA61K31/7088A61K31/713A61P7/10A61P11/02A61P11/06A61P17/04A61P27/02A61P27/04A61P27/06A61P27/14A61P29/00A61P43/00C12N15/1137C12N15/1138C12N2310/14A61K48/00C07H21/02C07H21/04C12N2310/346C12N2310/351C12N2320/30
Inventor J·M·亚恩J·E·查特尔顿D·M·森基纳D·A·盖玛彻S·T·米勒
Owner ARROWHEAD RES CORP
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