Combination therapy for prostate cancer using botanical compositions and bicalutamide

A prostate cancer and composition technology, applied in the direction of drug combination, plant raw materials, plant/algae/fungus/moss components, etc., can solve the problems of prostate cancer that cannot be cured

Inactive Publication Date: 2013-09-25
GENYOUS BIOMED INT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Bicalutamide along with LHH can help prevent cancer cel

Method used

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  • Combination therapy for prostate cancer using botanical compositions and bicalutamide
  • Combination therapy for prostate cancer using botanical compositions and bicalutamide
  • Combination therapy for prostate cancer using botanical compositions and bicalutamide

Examples

Experimental program
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Effect test

example 1

[0114] Example 1: Method for preparing plant extract

[0115] Compositions of the invention may be administered as a dried herb. Herbal preparations contain phytochemicals, some of which are soluble in aqueous media while others are relatively more soluble in organic (alcoholic, lipid) media. For the ability to extract active ingredients from medicinal herbs, different extraction methods were used and tested. Extraction methods include: aqueous (hot water) extraction; organic (lipid fraction) extraction; non-alcoholic organic (ethyl acetate) extraction; and alcoholic (ethanol) extraction.

[0116] The product is prepared from the herb or herb blend by extraction with a solvent (hot water, 80% ethanol or ethyl acetate) at reflux for 30-60 minutes, separated by filtration to obtain a filtrate, and air-dried for further use. analyze. These filtrates were combined, diluted or concentrated prior to determination of activity.

example 2

[0117] Example 2: OMN54 against prostate cancer cells and (bicalutamide) combination

[0118] OMN54 with The combination of (bicalutamide) achieved a greater anti-tumor growth effect than OMN54 alone or bicalutamide in C4-2 castration-resistant prostate cancer cells in vitro ( figure 1 ). Treatment with either bicalutamide (10 μM) or OMN54 (10 μg / ml) alone had minimal inhibitory effect on C4-2 cells, however, the combination of OMN54 (10 μg / ml) and bicalutamide (10 μM) at 48 h Significantly inhibited cell growth (see figure 1 ).

[0119] Effect of these two drugs and their combination on androgen receptor (AR) expression. AR protein expression was reduced in the OMN54-treated group, but not in the control group. Compared with OMN54 alone or bicalutamide group, in OMN54 and AR expression was further reduced in the (bicalutamide) combination group (see figure 2 ).

example 3

[0120] Example 3: Effect of OMN54 and bicalutamide treatment on AR expression

[0121] figure 2 The effect of OMN54 and bicalutamide treatment on AR expression in C4-2 cells is shown by Western blot analysis.

[0122] Using adult plant experiments, it was found that OMN54 and The combination of (bicalutamide) achieved a greater anti-tumor growth effect than OMN54 alone or bicalutamide in C4-2 castration-resistant human prostate cancer cells in vitro ( image 3 ). In this experiment, treatment with bicalutamide (20 μM) proved to have no effect, while OMN54 (50 μg / ml) showed significant inhibitory effect on C4-2 cells, however, OMN54 (50 μg / ml) combined with bicalutamide The combination of amines (20μM) inhibited cell growth even at 24h (see image 3 and Figure 4 ).

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Abstract

Botanical compositions comprising non-alcoholic organic extracts of Ganoderma lucidum, Salvia miltiorrhiza, and Scutellaria barbata for use in conjunction with bicalutamide therapy fpr cancer therapy, are provided. Methods for treatment or therapy of prostate cancer in a human is provided, the method comprising: administering an effective amount of a botanical composition that is effective for reducing androgen receptor protein expression; and administering concurrently an effective amount of a compound having anti-androgen activity, wherein the concurrent administration of the compound and the botanical composition achieves a therapeutic effect that is more effective than either agent alone.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application Serial No. 61 / 410,327, entitled "BOTANICAL COMPOSITIONS AND BICALUTAMIDE FOR PROSTATE CANCER," filed November 4, 2010, The content of this patent application is hereby incorporated by reference in its entirety. technical field [0003] The present invention relates generally to the field of compositions useful in the treatment of cancer. More precisely, the present invention provides a multifunctional multitargeted composition of plant extracts in combination with bicalutamide for the prevention and treatment of cancer, and in particular prostate cancer. Background technique [0004] Prostate cancer is cancer that develops in the tissue of the prostate gland (a gland found in the male reproductive system below the bladder and in front of the rectum). Prostate cancer usually occurs in older men. According to the National Cancer Institute, an esti...

Claims

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Application Information

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IPC IPC(8): A61K36/539A61K36/537A61K36/074A61P13/08A61P35/00A61K31/277
CPCA61K36/074A61K36/537A61K36/539A61K31/277A61K38/24A61K9/2086A61P13/08A61P35/00A61K2300/00
Inventor J·道杰弗里·道A·C·高W·格尔维克L·格尔维克M·A·乔丹J·米柯维茨T·奥克尼娃E·奥若杰夫
Owner GENYOUS BIOMED INT
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