Identification method of deer blood active crystal preparation

Abstract

The invention discloses an identification method of a deer blood active crystal preparation. The method comprises a PCR amplification test identification of specific DNA sequence of deer blood in the deer blood active crystal preparation, if a 405 bp stripe is amplified out, indicating that the preparation contains components of sika deer blood and red deer blood, if a 317 bp stripe is amplified out, indicating that the preparation contains components of sika deer blood; an immunoprecipitation test identification, if an immunoprecipitation line appears between each non-deer blood antibody immune serum and the deer blood active crystal preparation, indicating that the tested deer blood active crystal preparation contains other non-deer blood components; and an SDS-PAGE test identification for specific protein components contained in the deer blood active crystal preparation, if the processed gel appears feature spectrum bands, indicating that the deer blood active crystal preparation contains deer blood specific protein components. The method mentioned above provides a complete and scientific identification method for distinguishing true or false and high-quality or low-quality of deer blood crystal products in present markets.

Description

technical field [0001] The present invention relates to a method for identification of deer blood products, in particular to a method for identification of specific DNA sequences, specific antigenic determinants and specific protein components contained in active crystal preparations of deer blood. The specific identification of immunology and biochemistry belongs to the technical field of biological product identification. Background technique [0002] Deer-derived animal medicinal materials are rare and precious Chinese medicinal materials, among which medicinal materials derived from sika deer and red deer are listed as genuine products by the "Chinese Pharmacopoeia". Among various deer-derived medicinal materials, deer blood has been widely recorded and used in traditional Chinese medicine because it has the effects of tonifying deficiency, benefiting essence and blood, strengthening the waist and kidneys, and calming the mind. At present, the common deer blood products...

AI Technical Summary

Problems solved by technology

At present, the common deer blood products are mainly deer blood active crystals (deer blood crystals), but the fake deer blood crystal products on the market are often mixed with sheep blood, cow blood, horse blood, pig blood, donkey blood and dog blood, etc. Even the above-mentioned common animal blood is shoddy, so there is an urgent need for a series of scientific and reliable methods for detecting pure deer blood crystal products

At present, there is no complete scientific identification method for deer blood crystal products, especially in the identification of molecular biology, immunology and biochemistry of deer blood crystals. Characteristic identification methods in biology, immunology and biochemistry have become an urgent task at present

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