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Periploca forrestii schltr extract, preparation method and use thereof
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A kind of extract, the technology of the black bone rattan, applied in the field of black bone rattan extract and its preparation, can solve the problems of large amount of excipients, saving, inconvenient use of patients, etc., and achieve good anti-inflammatory effect
Inactive Publication Date: 2013-12-25
SICHUAN NORMAL UNIVERSITY
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[0004] The modern research on S. chinensis has also found that its medicinal activity is good, but the active ingredient content in its extract is low. If it is prepared into a prescription,
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[0029] Example 1 Preparation of the extract of the black bone vine of the present invention
[0030] After crushing the black bone vine, extract with 95% v / v ethanol, filter, repeat 3 times, the filtrate is dried under reduced pressure into an extract, and the extract is combined. Disperse the 95% ethanol extract evenly in about 2L of distilled water, shake and shake, and extract with equal volumes of petroleum ether and ethyl acetate. The ethyl acetate extract was dried under reduced pressure to obtain the ethyl acetate extract.
[0031] Take the ethyl acetate extract and mix the sample with 150 g of 160-200 mesh silica gel. The sample was applied to a silica gel column and eluted with a chloroform-methanol system. The elution ratios were pure chloroform, chloroform: methanol = 10:1, and chloroform: methanol = 5:1. Wash 4 column volumes for each gradient, take chloroform:methanol=5:1 elution position, remove the solvent, and dry to obtain the extract of Black bone vine.
[0032] ...
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[0043] Example 2 Screening of the effective parts of the black bone vine
[0044] 2.3 Extraction, separation and purification of the effective components of Heiguteng anti-rheumatoid
[0045] 2.3.1 Crude extraction of the effective components of Heiguteng anti-rheumatoid
[0046] Method 1: Crush the black bone vine with a pulverizer, reflux extraction with 50% ethanol, reflux extraction with a solid-liquid ratio of 10:1, reflux at 80°C for one hour, filter after reflux extraction, and repeat reflux extraction Once, filter, combine the filtrate and dry under reduced pressure at 60°C for use.
[0047] Method 2: After crushing the black bone vine, extract with 95% ethanol, repeat 3 times, dry the filtrate under reduced pressure at 50°C into an extract, and combine the extract. Take about 3g of the extract, spread it flat in a petri dish, dry it to constant weight at 105°C, parallel 3 times, and calculate the water content.
[0048] Disperse the 95% ethanol extract evenly into about 2L of...
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Abstract
The present invention provides a periploca forrestii schltr extract preparation method, which comprises: (1) taking a periploca forrestii schltr herb, crushing, extracting with ethanol with a volume ratio of more than 90%, and drying the extract to obtain an ethanol concrete; (2) taking the ethanol concrete, adding water to disperse, extracting by sequentially adopting petroleum ether and ethyl acetate, mixing the ethyl acetate layer, and removing the solvent to obtain an ethyl acetate concrete; and (3) taking the ethyl acetate concrete, adding to a silica gel column, sequentially carrying out gradient elution by adopting chloroform and methanol according to a ratio of (10-5):(0-1), collecting the elution segment at a ratio of chloroform to methanol of 5:1, removing the solvent, and drying to obtain the periploca forrestii schltr extract. The present invention further provides the extract prepared through the method, and a use thereof. The periploca forrestii schltr extract has characteristics of good anti-inflammatory activity, good anti-rheumatoid arthritis activity, significantly increased efficacy activity superior to other extraction sites, and no significant rebound after drug withdrawal, and provides a new selection for clinical medication.
Description
technical field [0001] The present invention relates to a kind of black bone vine extract and its preparation method and use Background technique [0002] Rheumatoid arthritis (Rheumatioid Arthritis, RA) is an autoimmune deficiency disease characterized by chronic inflammation of the joint synovium. In traditional Chinese medicine, it belongs to the category of "bi syndrome". , infection, immune disorders and other factors. The incidence of RA in my country is about 0.4%, mostly concentrated in southern provinces. Therapeutic methods include chemical medicine, traditional Chinese medicine, biological agents and other drug treatments and physical therapy. [0003] Black bone vine is the dry root or whole plant of Periploca forrestii Schltr, a plant of the genus Periploca Linn. in the family Asclepiasaceae. It is a commonly used medicine widely used in the treatment of rheumatism and rheumatoid, closed soft tissue injury and other diseases among the people. [0004] The mo...
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