Method for inducing test tube flowering of phalaenopsis
A test-tube flowering and phalaenopsis technology is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, and can solve the problems that flower buds are difficult to further develop into flowers, flower buds cannot be opened normally, and phalaenopsis cannot be achieved in vitro. The effect of normal and robust flower bud development, lasting flowering period, and simple and easy method
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Embodiment 1
[0016] The strong bottle seedlings of Phalaenopsis rooted by strong seedlings are used as induction materials for induction, which is carried out in two stages, one is the flower bud induction stage, and the other is the flowering cultivation stage.
[0017] (1) Flower bud induction stage: Take the strong bottle seedlings after the rooting of the strong seedlings as explants, and perform flower bud induction after removing the roots. A total of 15 strains were inoculated. The flower bud induction medium is: MS medium + 6-BA5mg / L, and 20g / L sucrose, 3g / L peptone, 150ml / L coconut milk, 0.5g / L activated carbon, 6g / L agar, pH5. 8. And adjust the amount of N and P in the MS medium, N is reduced to 1 / 5 of the original, and P is increased to 2 times of the original. The culture temperature was 18°C. The light time is 12h, and the light intensity is 30μmol m -2 the s -1 . Induced for 3 and a half months.
[0018] (2) Flowering cultivation stage: After the flower bud induction i...
Embodiment 2
[0021] Take the Phalaenopsis strong bottle seedlings that have taken root from the strong seedlings as the induction material and carry out the following steps to induce.
[0022] (1) Flower bud induction stage: Take the strong bottle seedlings after the rooting of the strong seedlings as explants, and perform flower bud induction after removing the roots. A total of 15 strains were inoculated. The flower bud induction medium is: MS medium + 6-BA7mg / L, and 30g / L sucrose, 4g / L peptone, 120ml / L coconut milk, 0.7g / L activated carbon and 7g / L agar, pH5. 7. And adjust the amount of N and P in the MS medium, N is reduced to 1 / 8 of the original, and P is increased to 3 times of the original. The culture temperature was 20°C. Lighting time 16h, light intensity 40μmol m -2 the s -1 . Induction for 4 months.
[0023] (2) Flowering cultivation stage: After the flower bud induction is completed, it can enter the flowering induction stage. The flowering induction medium is: 1 / 2MS m...
Embodiment 3
[0026] Take the Phalaenopsis strong bottle seedlings that are rooted through the strong seedlings as the induction material and carry out the following steps to induce.
[0027] (1) Flower bud induction stage: Take the strong bottle seedlings after the rooting of the strong seedlings as explants, and perform flower bud induction after root removal. A total of 15 strains were inoculated. The flower bud induction medium is: MS medium + 6-BA10mg / L, and 25g / L sucrose, 5g / L peptone, 100ml / L coconut milk, 1.0g / L activated carbon and 8g / L agar, pH5. 6. And adjust the amount of N and P in the MS medium, N is reduced to 1 / 10 of the original, and P is increased to 5 times of the original. The culture temperature was 16°C. Illumination time 16h, light intensity 50μmol m -2 the s -1 . Induction for 3 months.
[0028] (2) Flowering culture stage: the flowering induction medium is: 1 / 2MS medium is used as the basic medium, and sucrose 25g / L, peptone 5g / L, coconut juice 100ml / L, activat...
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