Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

DNA detection kit, and preparation method and applications thereof

A detection kit and reaction technology, which is applied in the field of DNA detection, can solve the problems of limited sensitivity improvement and achieve the effects of improved detection sensitivity, no impact on fluorescence performance, and simple operation process

Inactive Publication Date: 2014-01-15
SHENZHEN INST OF ADVANCED TECH
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The DNA probe based on single-particle quantum dots, combined with single-molecule detection technology, the detection of target DNA corresponds to the number of fluorescent bright spots of quantum dots. This method is relatively simple, has a high signal-to-noise ratio, and can perform trace detection, but The improvement of its sensitivity is mainly limited by the number of quantum dots and the number of target DNA molecules assembled, and the detection sensitivity can only reach 10 -15 mol / L

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA detection kit, and preparation method and applications thereof
  • DNA detection kit, and preparation method and applications thereof
  • DNA detection kit, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The target DNA includes target DNA1 and target DNA2, the sequences of which are respectively:

[0049] Target DNA1(HIV-1):5'-GCT ATA CAT TCT TAC TAT TTT ATT TAA TCC CAG-3'

[0050] Target DNA2 (HIV-2):5'-TAG ATTT AGT TGC GCCT GGT CCT TT-3'

[0051] Design a DNA detection kit according to the above-mentioned target DNA1 and target DNA2, specifically, in this embodiment, the DNA detection kit includes liposome-green quantum dot complex labeled detection DNA1, liposome-red quantum dot complex Object-labeled detection DNA2, magnetic bead-modified capture DNA1, magnetic bead-modified capture DNA2;

[0052] Wherein, the sequence numbers of detection DNA1, detection DNA2, capture DNA1 and capture DNA2 are:

[0053] Detection of DNA1: 5'-CTG GGA TTA AAT AAA A-A 10 -3'

[0054] Detection of DNA2: 5'-AAA GGA CCA GGC-A 10 -3'

[0055] Capture DNA1:5'-A 10 -TAG TAA GAA TGT ATA GC-3'

[0056] Capture DNA2:5'-A 10 -GCA ACT AAA TTC A-3'

[0057] In this embodiment, liposomes...

Embodiment 2

[0093] Target DNA only includes target DNA1, whose sequence is:

[0094] Target DNA1(HIV-1):5'-GCT ATA CAT TCT TAC TAT TTT ATT TAA TCC CAG-3'

[0095] Design a DNA detection kit according to the above-mentioned target DNA1, specifically, in this embodiment, the DNA detection kit includes liposome-green quantum dot complex-labeled detection DNA1, and magnetic bead-modified capture DNA1;

[0096] Among them, the sequence numbers of detecting DNA1 and capturing DNA1 are:

[0097] Detection of DNA1: 5'-CTG GGA TTA AAT AAA A-A 10 -3'

[0098] Capture DNA1:5'-A 10 -TAG TAA GAA TGT ATA GC-3’

[0099] In this embodiment, liposomes are formed from DSPC, DSPE-PEG-COOH, and cholesterol. In other embodiments, liposomes can be effectively broken and released under conditions such as organic solvents, ultrasound, and heat treatment. Quantum dots are other liposome-like structural materials.

[0100] The preparation method of above-mentioned DNA detection kit comprises:

[0101] (1) P...

Embodiment 3

[0111] Target DNA includes only target DNA2, whose sequence is:

[0112]Target DNA2 (HIV-2):5'-TAG ATTT AGT TGC GCCT GGT CCT TT-3'

[0113] Design a DNA detection kit according to the above-mentioned target DNA2, specifically, in this embodiment, the DNA detection kit includes liposome-green quantum dot complex-labeled detection DNA2, and magnetic bead-modified capture DNA2;

[0114] Among them, the sequence numbers of detecting DNA2 and capturing DNA2 are:

[0115] Detection of DNA2: 5'-AAA GGA CCA GGC-A 10 -3'

[0116] Capture DNA2:5'-A 10 -GCA ACT AAA TTC A-3'

[0117] In this embodiment, liposomes are formed from DSPC, DSPE-PEG-COOH, and cholesterol. In other embodiments, liposomes can be effectively broken and released quantum Point to other liposome-like structural materials.

[0118] The preparation method of above-mentioned DNA detection kit comprises:

[0119] (1) Preparation method of detection DNA2 labeled with liposome-red quantum dot complex

[0120] With ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
sizeaaaaaaaaaa
sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides a DNA detection kit. The DNA detection kit comprises detection DNA labeled by a liposome-quantum dot compound, and trap DNA modified by magnetic beads. According to the liposome-quantum dot compound, a plurality of individual particle quantum dots are enveloped by liposome. The detection DNA is bonded onto the surface of the liposome via amido bonds. According to the trap DNA modified by magnetic beads, the trap DNA is bonded onto the surface of the magnetic beads via amido bonds. Sequence of the detection DNA and sequence of the trap DAN are respectively capable of realizing complementation with sequences of the two ends of a target DNA. Sensitivity of the DNA detection kit is high, and can reach 10 to 18mol / L. The DNA detection kit can be used for simultaneous detection of multi-component DNA. The invention also provides a preparation method and applications of the DNA detection kit.

Description

technical field [0001] The invention relates to the field of DNA detection, in particular to a DNA detection kit and its preparation method and application. Background technique [0002] Among the existing DNA detection technologies, PCR-based target amplification technology is the most commonly used, but it requires a variety of primers, special DNA polymerase, and precisely controlled cycle temperature to separate DNA double strands. At present, some constant temperature amplification techniques have been gradually developed, such as rolling circle amplification, strand displacement amplification and loop-mediated isothermal amplification. These constant temperature amplification methods improve the amplification efficiency of target molecules, but still require some special Treatment methods and reaction conditions, such as pre-heat denaturation, padlock probe ligation, multiple primers and special DNA polymerases, etc., so these methods are more complicated and costly. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6816C12Q2537/125C12Q2563/107
Inventor 张春阳周娟王强心
Owner SHENZHEN INST OF ADVANCED TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com