Tricholomataceae fungi culture medium and preparation method and application thereof

A culture medium preparation and culture medium technology are applied in the field of white mushroom culture medium and preparation thereof, and can solve the problems of low utilization rate of starch and the like

Inactive Publication Date: 2014-04-16
魏晓辰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Armillaria also has the p

Method used

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  • Tricholomataceae fungi culture medium and preparation method and application thereof
  • Tricholomataceae fungi culture medium and preparation method and application thereof
  • Tricholomataceae fungi culture medium and preparation method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0031] This example provides a culture medium for the cultivation of fungi of the family Brassicaceae.

[0032] It contains 0.4% linoleic acid, 1.6% sucrose, 16.3% potato and the balance water by weight of raw material.

[0033] The preparation method of above-mentioned culture medium, comprises the following steps:

[0034] In this embodiment, the potatoes are peeled potatoes. The potatoes are put into the water for boiling, and after the potatoes are boiled, they are filtered with eight layers of gauze to obtain a filtrate. Mix the filtrate with the linoleic acid and sucrose, and use a rotary shaker to mix the raw materials evenly at a speed of 150r / min. After forming a stable, non-layered medium stock solution, divide the medium stock solution into 200ml Each part; the aliquoted culture medium stock solution was treated with high-pressure steam at 120°C and 102.9 KPa for 20 minutes, which was the culture medium for Armillaria armillaria in this embodiment.

Embodiment 2

[0036] This embodiment provides a culture medium for fungi of the family of white mushrooms.

[0037] It contains 0.01% linoleic acid, 2% sucrose, 10% potato and the balance water by weight of raw material.

[0038] The preparation method of above-mentioned culture medium, comprises the following steps:

[0039] In this embodiment, the potatoes are peeled potatoes. The potatoes are put into the water for boiling, and after the potatoes are boiled, they are filtered with eight layers of gauze to obtain a filtrate. Mix the filtrate with the linoleic acid and sucrose, and use a rotary shaker to mix the raw materials evenly at a speed of 160r / min. After forming a stable, non-layered medium stock solution, divide the medium stock solution into 150ml Each part; the aliquoted culture medium stock solution was treated with high-pressure steam at 125°C and 102.9 KPa for 15 minutes, which was the culture medium for Armillaria armillaria in this embodiment.

Embodiment 3

[0041] This embodiment provides a culture medium for fungi of the family of white mushrooms.

[0042] It contains 20% linoleic acid, 1% sucrose, 20% potato and the balance water by weight of raw materials.

[0043] The preparation method of above-mentioned culture medium, comprises the following steps:

[0044] In this embodiment, the potatoes are peeled potatoes. The potatoes are put into the water for boiling, and after the potatoes are boiled, they are filtered with eight layers of gauze to obtain a filtrate. Mix the filtrate with the linoleic acid and sucrose, and use a rotary shaker to mix the raw materials evenly at a speed of 100r / min. After forming a stable, non-layered medium stock solution, divide the medium stock solution into 200ml Each part; the aliquoted culture medium stock solution was treated with high-pressure steam at 120°C and 102.9 KPa for 20 minutes, which was the culture medium for Armillaria armillaria in this embodiment.

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Abstract

The invention discloses a tricholomataceae fungi culture medium and a preparation method and application thereof. The tricholomataceae fungi culture medium comprises polyunsaturated fatty acids which account for 0.01-20% of total weight of raw materials of the culture medium. The polyunsaturated fatty acids are added to the culture medium, and the dosage and the variety of the polyunsaturated fatty acids are optimized; the growth of tricholomataceae fungi can be effectively facilitated by the polyunsaturated fatty acids in the culture medium, especially a linoleic acid and a linolenic acid in the polyunsaturated fatty acids have especially obvious acceleration on growth of small genus armillaria and armillaria fungi, the starch utilization rate and the growth rate of armillaria mellea or halimasch can be increased by adopting the culture medium added with the linoleic acid, the biomass amplification can be up to more than four times in cultivation employing an ordinary culture medium, and the production of metabolites with a medicinal value is also obviously increased.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a culture medium for fungi of the family of mushrooms and its preparation method and application. Background technique [0002] Agaricaceae fungi belong to the class Agaricaceae and are a class of widely distributed fungi. There are a large number of economically valuable varieties of white mushrooms, most of which have delicious fruiting bodies, and some varieties have high medicinal value, such as the fungus of the genus Ordella, fungus of the genus Flammulina, fungus of the genus Calici, and Umberella Fungi, Armillaria, Armillaria fungi, etc. The research on the cultivation of Pleurotus officinalis has good economic prospects. [0003] Pseudoarmillaria ( Armillariella tabescens (Scop. : Fr.) Sing. ) as an example, Armillaria armillaria is a unique species in Asia, which can be used as both medicine and food. According to traditional Chinese medicine, the...

Claims

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Application Information

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IPC IPC(8): C05G3/00A01G1/04
Inventor 魏晓辰
Owner 魏晓辰
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