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Method for culturing Acanthopanax senticosus tetraploid

A cultivation method and technology of Acanthopanax senticosus, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of the yield of Acanthopanax senticosus and the low content of Acanthopanax senticosus, to improve the yield of medicinal materials and the method is simple Easy to implement, high mutagenicity effect

Inactive Publication Date: 2014-04-30
程钰翔
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved in the present invention is: aiming at the defects of the existing Acanthopanax output and the content of Acanthopanax glycosides are relatively low, a method for cultivating Acanthopanax tetraploids is proposed to improve the output of Acanthopanax senticosus and the content of Acanthopanax glucoside content

Method used

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Embodiment Construction

[0010] The present invention will be further described below in conjunction with specific examples.

[0011] The cultivation method of Acanthopanax tetraploid comprises the following steps:

[0012] (1) Pretreatment stage: Select well-developed, healthy and plump common diploid Acanthopanax seeds, wash them with tap water for 60 minutes, disinfect them with 70% alcohol on a sterile operating table for 4 minutes, rinse them with sterile water for 4 times, and then rinse them with tap water for 60 minutes. Disinfect with mercuric chloride with a concentration of 0.1% for 15 minutes, and finally rinse with sterile water for 6 times;

[0013] (2) Germination stage: the Acanthopanax senticosus seeds pretreated in step (1) are evenly distributed on the germination bed at a temperature of 25°C, keeping a certain distance between each other, using an appropriate amount of purified water as the germination agent, purified water It is advisable to half-soak the seeds until the seed bud...

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Abstract

The invention relates to a method for culturing Acanthopanax senticosus tetraploid. An aqueous solution of 0.01% colchicine and 0.3% dimethyl sulfoxide is used as an inducer to induce Acanthopanax senticosus seeds to obtain Acanthopanax senticosus tetraploid plant. The inducing method has Acanthopanax senticosus survival rate of 91.3%, and inductivity of 84.6%. The root, stem, leaf, plant height and the like of tetraploid Acanthopanax senticosus are all significantly better than original diploid Acanthopanax senticosus, so as to greatly increase the yield of medicinal material, and the eleutheroside content in tetraploid Acanthopanax senticosus is increased by 20%, compared that in the original diploid Acanthopanax senticosus. The method performs genetic improvement on Acanthopanax senticosus, and for the culture method of tetraploid Acanthopanax senticosus, colchicine and dimethyl sulfoxide are used to mutagenize the seeds. The method is simple and practicable, has high induction rate, and can be used for polyploidy seed selection, so that polyploidy breeding becomes an important method for genetic improvement and new variety breeding of Acanthopanax senticosus.

Description

technical field [0001] The invention relates to a method for cultivating medicinal plants, in particular to a method for cultivating tetraploid Acanthopanax. Background technique [0002] Acanthopanax is a shrub belonging to Araliaceae, and its roots, stems, leaves, bark, and fruits can be used as medicine. Acanthopanax has the functions of regulating the disorder of the body, nourishing the middle, benefiting the essence, strengthening the will, dispelling rheumatism, strengthening the muscles and bones, promoting blood circulation and removing blood stasis, invigorating the stomach and diuresis. Eleutheroside, an important ingredient in Acanthopanax, is a good tonic, which can increase the defense function of the body, and has the effects of anti-fatigue and preventing memory decline. Medicines and foods made from Acanthopanax senticosus are popular at home and abroad, but the predatory mining in the past two decades has severely damaged wild Acanthopanax senticosus resou...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 程钰翔
Owner 程钰翔
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