Sutai pig common disease-resistance combined selection index selecting breeding method

A comprehensive selection index and selective breeding technology, applied in animal husbandry and other directions, can solve the problems such as the evaluation system and selection of the general disease resistance of Sutai pigs that have not yet been established, and achieve the goal of accelerating the progress of disease resistance breeding, simple operation and high accuracy. Effect

Inactive Publication Date: 2014-07-23
YANGZHOU UNIV
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Problems solved by technology

However, so far, an evaluation system for the general disease resistance of Sutai pigs has not been established, so it is impossible to s

Method used

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  • Sutai pig common disease-resistance combined selection index selecting breeding method
  • Sutai pig common disease-resistance combined selection index selecting breeding method
  • Sutai pig common disease-resistance combined selection index selecting breeding method

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Embodiment 1

[0020] 1. Determination of general disease resistance indexes (important cytokines) of Sutai pigs

[0021] Blood was collected from the anterior vena cava of 180 piglets (60 in each group) at the age of 35, placed at 4°C for 30 minutes, and serum was routinely separated. Use the corresponding buffer solution to carry out four-fold eight-point concentration gradient dilution of the standard sample in turn to establish a standard curve. At the same time, dilute the sample serum with the corresponding buffer solution, process the sample serum with the porcine multi-cytokine detection kit (Affymetrix, USA) according to the operating instructions, and send it into the calibrated Bio-plex suspension chip system to read the corresponding fluorescence Then the concentration of cytokines (IL-2, IL-4, IL-6, IL-10, IL-12 and IFN-β) in the sample was calculated by the standard curve.

[0022] 2. Principal Component Analysis

[0023] Using the Factor module of SPSSl6.0 statistical softwa...

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Abstract

The invention relates to a sutai pig common disease-resistance combined selection index selecting breeding method. The method comprises the steps that front-cavity venous blood of 35-day-old piglets is collected, concentration of interleukin 2(IL-2), concentration of interleukin 4(IL-4), concentration of interleukin 6(IL-6), concentration of interleukin 10(IL-10), concentration of interleukin 12(IL-12) and concentration of beta interferon (IF-N-beta) are measured after serum samples are obtained, test pig groups are grouped at the same time, then data of the six indexes are converted into standardization Z values, the standardization Z values are substituted into a built combined selection index formulation to calculate a combined selection index value of each pig group, and individuals having higher disease resistance generally are determined according to the values. The detection method is easy to operate, high in accuracy, and capable of achieving automatic direct detection, and will give great play to disease-resistance breeding practice of the pigs.

Description

technical field [0001] The invention relates to the field of pig disease-resistant breeding, in particular to a selective breeding method for establishing a general disease-resistant comprehensive selection index of Sutai pigs and screening individuals with high disease resistance. Background technique [0002] In recent years, with the continuous acceleration of the scale of pig breeding, the simple pursuit of high yield usually leads to the reduction of disease resistance of pigs. Some original diseases have not been eradicated, and new diseases have emerged, which seriously restricts the development of pig industry. Diseases can lead to inferior pork products and reduced yields, while it also severely reduces genetic progress, causing direct economic losses to the swine industry of about 12%-15% of the total output value. Therefore, disease prevention is an important prerequisite for the healthy and rapid development of the pig industry. Traditional disease prevention an...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 吴圣龙包文斌朱世平蔡家嘉霍永久朱国强喻礼怀黄雪根华金第
Owner YANGZHOU UNIV
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