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Preparation method of intestinal targeting contrast agent

A technology for targeting contrast agents and intestines, applied in the field of nano-biomedical materials, can solve the problems of non-targeting, and achieve the effect of avoiding poor targeting, satisfying production and application, and simple process

Inactive Publication Date: 2016-06-15
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the contrast agents in clinical application are non-specific extracellular fluid space contrast agents without targeting

Method used

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  • Preparation method of intestinal targeting contrast agent
  • Preparation method of intestinal targeting contrast agent

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Experimental program
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Effect test

Embodiment 1

[0023] 1. Synthesis of biscarboxyl polyethylene glycol modified ferric oxide nanoparticles:

[0024] 2.1gFe(acac) 3 , 7.9ml oleylamine and 24g dicarboxy polyethylene glycol HOOC-PEG-COOH (molecular weight 2000) were dissolved in 200ml diphenyl ether. The solution was stirred at 400 rpm under nitrogen for 2 hours to remove oxygen. Incubate at 80 degrees Celsius for 4 hours, then quickly boil the solution for over 30 minutes. After the solution was cooled, it was washed three times with ether and ethanol, respectively. After adding PBS buffer to disperse uniformly for use.

[0025] 2. Antibody modification:

[0026] Dissolve 2 mg of the above ferric oxide particles in 0.01M PBS buffer solution, add 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC, 2.5mM) and N-hydroxyl Succinimide (NHS, 6.25 mM), and after about 15 minutes, 10 mL of TLR4 antibody (0.1 mg / ml) was added. React at 4 degrees Celsius for more than 10 hours, then centrifuge at 12,000 rpm to remove unreacted anti...

Embodiment 2

[0028] 1. Synthesis of ferric oxide nanoparticles modified by carboxylamino polyethylene glycol:

[0029] 1.6g FeCl 3 ·6H 2 O, 8.5ml oleic acid and 24g carboxyamino polyethylene glycol NH 2 -PEG-COOH (molecular weight 2000) was dissolved in 200 ml of diphenyl ether. The solution was stirred at 400 rpm under nitrogen for 2 hours to remove oxygen. Incubate at 80 degrees Celsius for 4 hours, then quickly boil the solution for over 30 minutes. After the solution was cooled, it was washed three times with ether and ethanol, respectively. After adding PBS buffer to disperse uniformly for use.

[0030] 2. Antibody modification:

[0031] Dissolve 2 mg of the above ferric oxide particles in 0.01M PBS buffer solution, add 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC, 2.5mM) and N-hydroxyl Succinimide (NHS, 6.25 mM), and after about 15 minutes, 10 mL of TLR4 antibody (0.1 mg / ml) was added. React at 4 degrees Celsius for more than 10 hours, then centrifuge at 12,000 rpm to rem...

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Abstract

The invention relates to a preparation method of an intestinal-targeting contrast agent, comprising the synthesis of ferric oxide nanoparticles modified by functional polymers and antibody modification. The advantage of the intestine-targeted contrast agent is that the nano-iron ferric oxide particles are used as a medium, and a specific antibody is connected to form a contrast agent with intestinal targeting, which can avoid the problem of poor targeting of commonly used contrast agents. The raw materials used have high biological safety, and some of them are already commercial products. The contrast agent prepared by the invention has good physical and chemical stability, biocompatibility and magnetic resonance imaging performance. The preparation method in the invention has simple process and strong operability, and can further meet production and application requirements.

Description

technical field [0001] The invention relates to a preparation method of an intestinal-targeted magnetic resonance contrast agent, in particular to a preparation technology of a contrast agent using superparamagnetic iron oxide as a medium and simultaneously connecting antibodies on the surface of macrophage cell membranes in inflammatory bowel disease. The invention belongs to the field of nanometer biomedical materials. Background technique [0002] Inflammatory bowel disease (IBD) is a group of intestinal inflammatory diseases whose etiology and pathogenesis are not fully understood. Magnetic resonance imaging (MRI) colonoscopy can be used to detect and quantify ulcerative colitis, reflecting colonic lesions and activity. On contrast-enhanced MRI, the degree of enhancement of the bowel wall correlates with the degree of inflammatory activity. Magnetic resonance imaging can be freely selected in the process of image acquisition, and has the advantage of providing spatiall...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/12
Inventor 何丹农王萍朱君颜娟夏智伟
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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