Tenascin-C and application thereof in rheumatoid arthritis

A technology for tenascin and arthritis, applied in the field of tenascin C and its use in rheumatoid arthritis, which can solve the problems of impractical direct assessment

Inactive Publication Date: 2014-10-15
OXFORD UNIV INNOVATION LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] Determination of bone erosion is based almost exclusively on imaging findings, ultr...

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  • Tenascin-C and application thereof in rheumatoid arthritis
  • Tenascin-C and application thereof in rheumatoid arthritis
  • Tenascin-C and application thereof in rheumatoid arthritis

Examples

Experimental program
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Embodiment Construction

[0072] Assessment of Tenascin C Levels in Patients with Rheumatoid Arthritis Compared to Normal / Control Individuals

[0073] Sera were obtained from the blood of patients with rheumatoid arthritis as well as from normal individuals and stored at -80°C until use. On the day of analysis, sera were thawed on ice and typically diluted 1:50 in EIA buffer (1% BSA, 0.05% Tween 20 in PBS) for use in ELISA. An ELISA kit was purchased from IBL (Catalog No. 27751) for the detection of high molecular weight variants of human tenascin C. 100 μL of diluted serum was incubated on ELISA plates pre-coated with an unlabeled capture anti-tenascin C antibody in duplicate at 37 °C for 60 min. The ELISA plate (in 0.001% Tween 20 / PBS) was washed 7 times, and HRP-labeled anti-human tenascin C antibody (100 μL) was added to each well. The wells were then incubated at 4°C for 30 minutes, and then washed 9 times with 0.001% Tween 20 / PBS. 100 μL of chromogen (TMB solution provided) was then adde...

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Abstract

The present invention provides a method of determining the rheumatoid arthritis status of a subject, or the progression of rheumatoid arthritis, or the appropriate treatment for a subject with rheumatoid arthritis, comprising the steps of (a) determining the level of tenascin-C in a sample from said subject; and (b) comparing the level of tenascin-C determined in step (a) with one or more reference values. Preferably the rheumatoid arthritis referred to is erosive rheumatoid arthritis. The invention, when being disclosed, is accompanied with Figure 5.

Description

technical field [0001] The present invention relates to the use of tenascin-C as a biomarker for inflammatory disorders, in particular as a serum biomarker for rheumatoid arthritis. In particular, the present invention relates to the use of tenascin C as a biomarker for erosive rheumatoid arthritis. Background technique [0002] Tenascin C is a proinflammatory extracellular matrix glycoprotein (Midwood, et al. (2009), Nat. Med., 15:774-780), which is activated by the pattern recognition receptor TLR4 Induction of inflammatory cytokine synthesis in primary human macrophages and synovial fibroblasts. Tenascin C is a larger hexameric protein with a molecular weight of 1.5 million Daltons. High levels of tenascin C have been found at sites of inflammation such as in the synovial fluid of rheumatoid arthritis patients and in the tumor stroma. [0003] Rheumatoid arthritis is a chronic disease characterized by persistent inflammation, swelling and pain in multiple joints. Over...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/564
CPCC07K16/241G01N33/6893G01N33/564C07K16/2887G01N2333/78G01N2800/102C07K16/248A61K31/519A61P19/00A61P19/02A61P29/00
Inventor 金·苏姗妮·米德伍德
Owner OXFORD UNIV INNOVATION LTD
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