A kind of synthetic method of medroxyprogesterone artificial antigen

A technology of medroxyprogesterone and artificial antigen, which is applied in the field of synthesis of medroxyprogesterone artificial antigen, can solve the problems of expensive instruments, time-consuming detection, cumbersome pretreatment process and the like, and achieves the effect of simple synthesis process

Active Publication Date: 2016-08-24
无锡迪腾敏生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have accurate detection results and high sensitivity, but the whole process requires professional and technical personnel to operate, the equipment used is expensive, the sample pretreatment process is cumbersome, and the detection is time-consuming, which cannot meet the needs of rapid detection in large quantities on site.

Method used

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  • A kind of synthetic method of medroxyprogesterone artificial antigen
  • A kind of synthetic method of medroxyprogesterone artificial antigen
  • A kind of synthetic method of medroxyprogesterone artificial antigen

Examples

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Embodiment 1

[0026] (1) Preparation of medroxyprogesterone hapten:

[0027] Dissolve 34.4mg (0.1mmol) of medroxyprogesterone in 3mL of anhydrous dimethyl sulfoxide as solution A, dissolve 27.8mg (0.2mmol) of dibromoacetic acid in 1.4mL of anhydrous dimethyl sulfoxide as solution B and A Add 208.5 mg (1.5 mmol) of potassium hydroxide powder to the solution, stir at room temperature until the color of the solution turns slightly yellow, add solution B to solution A drop by drop, the yellow color gradually deepens, bromine replaces the 17th hydroxyl group of medroxyprogesterone The H bond of the carboxyl group becomes the medroxyprogesterone hapten, react at room temperature for 4 hours, stop the reaction with 50mL ice water, extract three times with ethyl acetate, combine the water phase, and acidify the water phase with 2mol / L hydrochloric acid to obtain a yellow precipitate , washed four times with water until neutral, and dried to obtain yellow medroxyprogesterone hapten (MP-2CME) powder....

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Abstract

The invention relates to a synthetic method of medroxyprogesterone artificial antigen, which belongs to the technical field of biochemical engineering. According to the invention, medroxyprogesterone is taken as a raw material, substitution is carried out through potassium hydroxide and dibromoacetic acid to obtain medroxyprogesterone semiantigen, and a mixed anhydride method is used for coupling the medroxyprogesterone semiantigen with carrier protein BSA to obtain the medroxyprogesterone artificial antigen. The method of the invention successfully synthesizes the medroxyprogesterone artificial antigen, the synthesis process is simple, the antigen products expose common structure of progestogen, a base is established for screening a mass selection medroxyprogesterone monoclonal antibody, and the obtained mass selection medroxyprogesterone monoclonal antibody can satisfy the requirement of multiple detection to progestogen in our country.

Description

technical field [0001] The invention relates to a method for synthesizing an artificial antigen of medroxyprogesterone, belonging to the technical field of biochemistry. Background technique [0002] Medroxyprogesterone (Medroxyprogesterone, MP) belongs to the progestin, is a synthetic progesterone derivative, the effect is similar to natural progesterone, also known as medroxyprogesterone. It is a kind of progestational agent. It is mainly used in medicine for dysmenorrhea, functional amenorrhea, habitual abortion, etc. It can be used as a long-acting contraceptive injection in large doses. In farming, it is mainly used to promote animal growth. However, the metabolism in animals is slow, and it is easy to remain, and it is easy to cause sexual dysfunction, poor mental state, and even cause liver and kidney damage, induce tumors and other potential hazards. The existing methods for detecting medroxyprogesterone are instrumental detection, including high performance liquid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/765C07K14/77
CPCC07K14/765C07K14/77C07K19/00
Inventor 胥传来孔娜匡华徐丽广马伟刘丽强宋珊珊吴晓玲
Owner 无锡迪腾敏生物科技有限公司
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