Fluorescent microsphere marker and preparation method thereof

Abstract

The invention relates to a fluorescent microsphere marker and a preparation method thereof. The invention aims to provide a fluorescent microsphere marker. Compared with the traditional fluorescent marker, the fluorescent microsphere marker disclosed by the invention has the advantages of higher fluorescence intensity and higher stability, is easier to mark and is simpler to prepare. The technical scheme of the fluorescent microsphere marker is to provide the fluorescent microsphere marker. The fluorescent microsphere marker comprises a carrier, wherein the carrier is a microsphere polymerized by a styrene monomer, and the microsphere is connected with a fluorescence complex. The fluorescent microsphere marker disclosed by the invention has the beneficial effects that the preparation method is simpler and easier because the microsphere polymerized by the styrene monomer is used as the carrier. The microsphere disclosed by the invention not only can be used for marking the rare earth fluorescence complex but also can be used for marking common fluorescence substance, such as FITC (fluorescein isothiocyanate), the microsphere with various fluorescence properties can be manufactured, and the requirement of multiple detection can be satisfied.

Description

technical field [0001] The invention relates to a fluorescent microsphere marker and a preparation method thereof. Background technique [0002] Commonly used fluorescent markers, such as fluorescein isothiocyanate (FITC), rhodamine-6-G (R-6-G), etc., have short fluorescence lifetimes and are difficult to overcome the interference of background fluorescence, so the detection sensitivity is low. The use of rare earth complexes with long fluorescence lifetimes as markers and time-resolved detection technology can effectively overcome the interference of background fluorescence and greatly improve the sensitivity. This technology is called time-resolved fluorescence detection technology, which has become one of the common technologies in biomedical research and clinical biochemical detection. [0003] Commonly used rare earth ions mainly include: europium, samarium, dysprosium and terbium, which can form complexes with strong fluorescence with certain specific complexes. Rare...

AI Technical Summary

Problems solved by technology

(3) Narrow emission band

(4) Larger Stokes displacement

[0008] Existing rare earth fluorescent complex markers, rare earth ions are mainly Eu 3+ 、Sm 3+ 、Dy 3+ and Tb 3+ The complexes formed with some organic complexes such as β-diketone compounds, o-phenanthroline compounds, salicylic acid compounds, bipyridine compounds, etc., have been used to varying degrees, but it is difficult to meet the requirements simultaneously. The following requirements: (1) have strong fluorescence