Construction method and application of restorer line of hybrid scallops of purple scallops and Argopecten irradians concentricus

A construction method and technology of a restorer line, applied in the field of shellfish breeding technology--the cultivation of hybrid scallop commercial seedlings, can solve problems such as the inability to establish male sterile lines, maintainer lines, and restorer lines, so as to overcome the obstacles of self-fertilization, Effects of low cost and increased yield

Active Publication Date: 2014-11-26
台州市金瓯海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in animal breeding, there have been no reports of male sterile and female fertile individuals at home and abroad so far, so it is impossible to establish male sterile lines, maintainer lines and restorer lines similar to crops for hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Breeding of Zimo hybrid scallop restorer lines: Select 50 large individual scallops from the male sterile line of Zimo hybrid scallops, fertilize them with sperm from different Gulf of Mexico scallops after promoting maturation and oviposition, and establish at least 20 backcrosses At the same time, the Gulf of Mexico scallops that provided sperm were self-propagated to establish an inbred family, and the growth and survival of the backcross progeny were measured, and the combining ability test was carried out; from the second year onwards, the traits were selected according to the results of the combining ability test and the development of gonads For a backcross family that is excellent and contains hermaphroditic scallops, the shellfish of the related male sterile family will be continuously backcrossed with the sperm of the Gulf of Mexico scallop hybrid family for sperm to establish a backcross family until all the backcross families are hermaphroditic and have goo...

Embodiment 2

[0024]In 2011, using the imported purple scallop and the Gulf of Mexico scallop from Zhanjiang, Guangdong, a first-generation orthogonal and a reciprocal family of interspecific hybrids were co-bred. According to actual measurements, the average shell height of Zimo hybrid scallops (the hybridization of purple scallop eggs and Gulf scallop sperm) is 52.38 mm, the weight is 27.40 grams, and the column weight is 2.46 grams. The body weight is 14.80 grams, and the column weight is 1.13 grams. Compared with the Gulf of Mexico scallop of the same period, the first generation of Zimo hybrids had a shell height of 24.7%, a body weight of about 85.1%, and a column weight of 117.7%. In the spring of 2012, after promoting the maturation of the first-generation scallops of the above-mentioned hybrids, select scallops that are large in size and only develop in the female area. After drying in the shade for 30 minutes, place the scallops in a container filled with seawater at 23°C to stimu...

Embodiment 3

[0029] In 2014, a total of 14 first-generation hybrid families of purple scallop eggs and Gulf scallop sperm were established using imported purple scallops and Gulf scallops, and self-bred families of Gulf scallops for sperm were established, according to the method in Example 1 The male sterile line of Zimo hybrid scallop and its corresponding maintainer and restorer lines can be bred within 1-3 years to realize the commercial production of Zimo hybrid scallop seedlings.

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PUM

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Abstract

The invention relates to a construction method and application of the restorer line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The restorer line is Argopecten irradians concentricus strains capable of enabling the generations of the male sterility line to restore the characteristic of male sterility, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are the hybrid generations of purple scallop ova and Argopecten irradians concentricus sperms. The construction method comprises the steps that excellent individuals in the male sterility line are selected, different varieties of Argopecten irradians concentricus are used for continuous backcross until all generations are hermaphrodite, and the restorer line of the male sterility line is obtained; finally, the restorer line and the corresponding male sterility line are used for hybridization to produce commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus. According to the construction method and application, the restorer line of the male sterility liner is firstly built in animals, the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated, and the yield of the produced hybrid scallops of the purple scallops and the Argopecten irradians concentricus is improved by more than 42 percent relative to that of the Argopecten irradians concentricus in the same year.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to a construction method and application of a restorer line of Zimo hybrid scallop (the hybrid offspring of purple scallop ova and Mexico gulf scallop sperm). Background technique [0002] The Gulf of Mexico scallop (Argopecten irradians concentricus) is native to the coast of the Gulf of Mexico in the United States and was introduced to my country in the 1990s. The Gulf of Mexico scallop grows fast and adapts to a wide range of temperatures. It is especially suitable for high-temperature sea areas and has strong stress resistance. It is very suitable for farming in the southern seas of my country. It is currently one of the main scallop breeding species in the southern seas. However, due to the long-term inbreeding after the introduction, the germplasm of the Gulf of Mexico scallop has been seriously degraded d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王春德孙奎府马斌
Owner 台州市金瓯海洋科技有限公司
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