A kind of portable probe type surface plasmon resonance biosensor and its preparation and detection method

Abstract

A portable penetrating surface plasmon resonance biosensor and its preparation and detection method, the sensor includes a strip of optical glass, the bottom of which is coated with a metal internal reflection film, the side wall is coated with a chromium film, and the nano-chromium film is coated with biological The gold film of the molecular sensitive layer, and the incident optical path of the strip optical glass is equipped with a collimator, and the outgoing optical path is sequentially equipped with a polarizer, an optical fiber collimator, and a fiber optic spectrometer connected to the optical fiber collimator through a multimode optical fiber. Metal internal reflection film is coated on the bottom of the strip optical glass; chrome film and gold film are sequentially plated on the side wall of the top; a biomolecular sensitive layer is formed on the surface of the gold film; and sealed with BSA solution to obtain a penetrating surface plasma Body resonance biosensors. The invention inserts the test tube into the liquid for SPR measurement, simplifies the system and realizes the portability of the sensor. At the same time, it overcomes the defects of the existing probe-type SPR sensor, and its measurement method is much simpler, realizing the portability of the instrument.

Description

technical field [0001] The invention relates to a surface plasmon resonance biosensor, in particular to a portable penetrating surface plasmon resonance biosensor and a preparation and detection method thereof. Background technique [0002] Surface plasmon resonance (surface plasmon resonance, SPR) is a physical optical phenomenon produced by light beam passing through the surface of the medium and coupled with the metal film. In 1902, Wood first discovered this phenomenon in optical experiments. One of the necessary conditions for the formation of SPR is the existence of the interface between the metal and the dielectric. When the parallel light on the parallel surface is incident at an angle greater than the critical angle of total reflection, if its frequency is consistent with the frequency of free electrons (ie, plasma) oscillating on the metal surface, The incident light will be coupled into the surface plasmon. At this angle, the surface plasmon resonance will cause ...

AI Technical Summary

Problems solved by technology

The existing problems of fiber optic SPR sensors are: the sensitivity and signal-to-noise ratio of multimode fiber SPR are not high, and the resonance wavelength is dispersive; while the core diameter of single-mode fiber is small, the coupling light is weak

In addition, the preparation process of fiber optic SPR sensing probes is more complicated than that of planar sensor sheets.

In order to overcome the above problems, a sensor probe (TakuoAkimoto, AnalyticaChimicaActa610 (2008) 119–124) processed with optical glass rods instead of optical fibers has appeared, but a beam splitter is required, and the reflected light has less than half of the light intensity Entering the spectrometer, the signal is lost by more than half

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