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In vitro separation system and in vitro separation method of acute myeloid leukemia cells and hematopoietic stem cells

A technology of hematopoietic stem cells and leukemia cells, which is applied in the field of in vitro separation system of acute myeloid leukemia cells and hematopoietic stem cells, can solve the problems of difficult and complicated separation, and achieve the effect of simple separation and simple separation

Active Publication Date: 2016-08-17
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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Problems solved by technology

However, these cancer cell isolation methods depend on the availability of tumor-specific antibodies, or the unique morphology of different cancer cells, making isolation complex and difficult

Method used

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  • In vitro separation system and in vitro separation method of acute myeloid leukemia cells and hematopoietic stem cells
  • In vitro separation system and in vitro separation method of acute myeloid leukemia cells and hematopoietic stem cells
  • In vitro separation system and in vitro separation method of acute myeloid leukemia cells and hematopoietic stem cells

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Embodiment Construction

[0026] The in vitro separation system and in vitro separation method of acute myeloid leukemia cells and hematopoietic stem cells will be further described in detail below mainly in conjunction with the accompanying drawings and specific implementation methods.

[0027] like figure 1 As shown, an in vitro separation system 100 for acute myeloid leukemia cells and hematopoietic stem cells according to an embodiment includes a sample storage device 110 , a separation component 120 and a collection device 130 .

[0028] The sample storage device 110 is used for holding the serum mixture containing acute myeloid leukemia cells and hematopoietic stem cells to be separated. Wherein, the serum mixture containing acute myeloid leukemia cells and hematopoietic stem cells is composed of acute myeloid leukemia cells, hematopoietic stem cells and serum. Wherein, the material of the sample storage device 110 may be transparent glass or transparent polymer material. The sample storage dev...

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Abstract

The invention discloses a system for in-vitro separation of acute myeloblastic leukemia cells and hemopoietic stem cells. The system comprises a sample storage device, a separation assembly and a collection device. The separation assembly comprises a housing, a mounting base and micropore pipes. The housing is provided with a receiving chamber, the mounting base is arranged in the receiving chamber, the receiving chamber is divided into a first chamber and a second chamber, the micropore pipes are fixedly arranged on the mounting base and communicate the first chamber and the second chamber, hemopoietic stem cells can go through the micropore pipes, the sample storage device is communicated with the housing by a pipe so that the sample storage device is communicated with the first chamber, and the collection device is connected to the housing by a pipe so that the collection device is communicated with the second chamber. The system for in-vitro separation of acute myeloblastic leukemia cells and hemopoietic stem cells realizes simple separation of acute myeloblastic leukemia cells and hemopoietic stem cells. The invention also provides a method for in-vitro separation of acute myeloblastic leukemia cells and hemopoietic stem cells.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an in vitro separation system and an in vitro separation method for acute myeloid leukemia cells and hematopoietic stem cells. Background technique [0002] Leukemia, also known as blood cancer, is a kind of malignant clonal disease of hematopoietic stem cells. Common leukemias include acute myelocytic leukemia (acute myelocytic leukemia, acute myeloid leukemia cells), acute lymphocytic leukemia (acute lymphoblastic leukemia, ALL), chronic myelocytic leukemia (chronic myelocytic leukemia, CML), chronic lymphocytic leukemia (chronic lymphocytic leukemia , CLL). The main pathological feature of leukemia is malignant and unlimited proliferation of leukemia cells in the bone marrow and other hematopoietic tissues, infiltrating various tissues and organs throughout the body, thereby directly affecting the functions of some important organs, and inhibiting normal hematopoiesis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N5/09C12N5/0789
CPCC12M47/04C12N5/0647C12N5/0694C12N2509/00
Inventor 唐斌颜庆云周卓龙葛永梅
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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