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The method for obtaining the diseased plants of T. dwarfis by indoor cultivation

The technology of a dwarf black powdery fungus and a cultivation method is applied in the field of indoor cultivation to obtain the diseased plants of the wheat dwarf black powder fungus, which can solve the problems of the spread of the bacteria, the limitation of research, the inability to clarify the environmental conditions, and the like, so as to strengthen the prevention and control and improve the infection rate. Activity, the effect of increasing the infection rate

Active Publication Date: 2017-01-18
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few relevant studies on the artificial inoculation test of TCK teliospores in China. In Dalian, Beijing, Tianjin and other places, a large number of TCK diseased plants were obtained by sprinkling TCK teliospores on the soil surface outdoors, but the research was carried out under outdoor conditions. It is easy to cause the spread of germs and cause serious consequences
Because it is difficult to cultivate diseased plants under indoor conditions, the domestic research on the infection cycle of T. dwarfis is limited, and the environmental conditions of each stage in the cycle of T. dwarf disease cannot be clarified.

Method used

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  • The method for obtaining the diseased plants of T. dwarfis by indoor cultivation
  • The method for obtaining the diseased plants of T. dwarfis by indoor cultivation
  • The method for obtaining the diseased plants of T. dwarfis by indoor cultivation

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Experimental program
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Effect test

Embodiment 1

[0041] Embodiment 1 TCK teliospore concentration influence research on germination

[0042] Materials and Methods

[0043] The tested strain was intercepted from the port in 2011, and it was detected by electron microscopy as Tilletia tritici. The inventor's laboratory is preserved, and can be released to the public for verification tests within 20 years from the date of application. The method of the present invention has no special requirements for strains, and other T. tritici strains can also be used to verify the present invention.

[0044] Take 1 gall from the diseased ear, put it in a 2mL centrifuge tube, add an appropriate amount of distilled water to soak the gall, let it stand for 30 minutes, then mash the gall and shake it fully, filter it through a 150-mesh sieve, and put the filtrate in the Cultivate at room temperature for 24 hours, discard the supernatant after low-speed centrifugation, add 0.25% NaClO aqueous solution and place at room temperature for 1 minut...

Embodiment 2

[0053] The indoor culture of embodiment 2 wheat dwarf Tilletia smut pathogenic plants

[0054] 1. Preparation of germinated teliospores of T. tritici

[0055] (1) Surface disinfection treatment of teliospores of T. dwarfis: Take 1 gall from the diseased ear, put it in a 2mL centrifuge tube, add appropriate amount of distilled water to soak the gall, let it stand for 30 minutes, and then mash the gall And shake fully, filter with 150 mesh sieve, incubate the filtrate at room temperature for 24 hours, discard the supernatant after low-speed centrifugation, add 0.25% NaClO aqueous solution and place it at room temperature for 1 min to carry out surface disinfection, wash with sterilized water for 3 The second time, use a hemocytometer to adjust the teliospores in sterilized water to (50-130)×104 / mL.

[0056] (2) Germination of teliospores of T. tritici: add the prepared teliospore suspension to the pre-made soil extract medium plate (1000mL medium contains 20g agar, 5mg 800,000 ...

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Abstract

The invention discloses a 'method for cultivating tilletia controversa kuhn disease plants in indoor environments', and belongs to the field of tilletia controversa kuhn control. The method includes steps of (1), preparing tilletia controversa kuhn germination winter spores; (2), preparing wheat seeds for tilletia controversa kuhn inoculation; (3), artificially inoculating wheat; (4), cultivating the disease plants in the indoor environments. The method for cultivating the tilletia controversa kuhn disease plants in the indoor environments has the advantages that the stable tilletia controversa kuhn morbidity can be acquired under indoor conditions, the method is beneficial to tilletia controversa kuhn biological research under the indoor isolation conditions, diffusion of germs during outdoor research can be prevented, and accordingly the method is favorable for controlling tilletia controversa kuhn diseases from various aspects.

Description

technical field [0001] The invention relates to a method for indoor culturing and obtaining the diseased plants of Tilletia tritici, belonging to the field of prevention and control of Tilletia tritici. Background technique [0002] Tilletia controversa Kühn (TCK) is an important international quarantine disease caused by Tilletia controversa Kühn (TCK), and it is also a dangerous plant disease in my country's foreign quarantine. The disease is very harmful and difficult to control. Once introduced, it will cause devastating damage to wheat production, and the incidence rate is usually approximately equal to the loss rate of wheat yield. According to Hoffmann's report, in popular years, the loss rate caused by TCK is generally 20% to 50%, and the highest can reach 75% to 90%. In addition to yield loss, pollination of infested wheat decreases with infection severity and quality is greatly affected by the presence of TCK teliospores. At present, the disease has occurred in a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G7/06C12N3/00
Inventor 高利姚卓陈万权刘太国刘博
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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