Method for separating and purifying 4,8-dht from pecan exocarp and application of 4,8-dht

Abstract

The invention discloses a method for separating and purifying 4,8-DHT from pecan exocarp and an application of 4,8-DHT. The method comprises the following steps: performing ultrasonic extraction to obtain a pecan exocarp extracting liquid by virtue of independent excitation type ultrasonic sounding, performing reduced pressure concentration, then passing through X-5 macroporous resin, performing gradient elution by using a mixed solution of water and ethanol, collecting an eluate obtained by eluting with a mixed liquid of water and ethanol in a volume ratio of 4 to 6, performing reduced pressure concentration to obtain a concentrated solution, performing silica-gel column chromatography, performing gradient elution by using a mixed solvent of petroleum ether and ethyl acetate, totally collecting 36 bottles of flow components by collecting a bottle of the flow components per 1 / 2 column volume, combining the tenth to eighteenth bottles of the flow components, performing reduced pressure concentration, and performing ethyl acetate crystallization to obtain a 4,8-dyhydroxy-1-tetralone crystalline compound. 4,8-DHT disclosed by the invention is of a 1:1 raceme, is less in impurity, has the purity of 99.5-99.8%, and ensures that the extraction rate reaches more than 94%; and the 4,8-DHT raceme has the effects on inhibiting seed germination and seedling growth of plants.

Description

technical field

[0001] The invention relates to the extraction of 4,8-dihydroxy-1-tetralone, in particular to a method for separating and purifying 4,8-dihydroxy-1-tetralone from the exocarp of hickory nut and the application of 4,8-DHT . Background technique

[0002] 4,8-dihydroxy-1-tetralone (4,8-DHT), chemical name 4,8-dihydroxy-1-tetralone, colorless fine cluster needle crystal, in which the 4th carbon connected to the hydroxyl group is a chiral carbon. The left-handed enantiomer (–) of 4,8-DHT is called regiolone and the right-handed enantiomer (+) is called isosclerone. Regiolone was first identified from green walnut and can be used to treat skin itching and pain. Isosclerone was first introduced from Sclerotinia sclerotium The compound was isolated and identified in , and later also isolated from some fungi, which can cause grape gray spot disease. Pecan exocarp also contains 4,8-DHT, which is a 1:1 racemate, melting point 366-368K, soluble in water, ethanol, ac...