A kind of method utilizing ethyl methanesulfonate to make Clivia strong seedling

A technology of ethyl methanesulfonate and Clivia, which is applied in the fields of seed and rhizome treatment, botanical equipment and methods, applications, etc., can solve problems such as the difficulty of Clivia seedlings, and achieve reduced cultivation and management difficulties, strong growth potential, and strong root system. developed effect

Inactive Publication Date: 2016-07-27
SHANGHAI ACAD OF AGRI SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for using ethyl methanesulfonate to make Clivia seedlings strong, aiming to solve the technical problem of Clivia's difficult seedling growth in the seedling cultivation process

Method used

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  • A kind of method utilizing ethyl methanesulfonate to make Clivia strong seedling
  • A kind of method utilizing ethyl methanesulfonate to make Clivia strong seedling

Examples

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Embodiment 1

[0032] Peel the seeds from the post-ripened Clivia grandiflora fruit, wash them with water, dry them in the air, remove the shriveled seeds, select seeds with full grains, normal development, and bud eyes, soak them in warm water at 28±2°C for 20 hours, take them out and drain Dry.

[0033] Under the above-mentioned same temperature conditions, put the above-mentioned seeds into 0.2% (v / v) EMS solution, shake for 4 hours on a shaking table with a set speed of 200 rpm, then take them out, wash them with running water for 4 hours, and absorb them. Dry surface moisture and set aside. The EMS solution is prepared with 0.1 mol / L, pH=6.8 phosphate buffer.

[0034] Using pine needles as substrate, the treated seeds are sown and managed according to conventional methods, and seedlings with a seedling height of 1.5 cm and a radicle length of about 3 to 4 cm can grow in 110 to 120 days, and the seed emergence rate is about 87.0%.

[0035] The seedlings with a height of about 1.5 cm ar...

Embodiment 2

[0038] The sowing and cultivation management of Clivia seeds are basically the same as in Example 1, only the treatment concentration and time of EMS have been changed. In this embodiment, the treatment concentration of EMS is 0.15% (v / v), and the treatment time is 6 hours. The emergence rate of the seeds in this embodiment is about 88.6%. After 1 year of sowing, an average of 4.8 blades can be grown and 6.0 roots can be grown; after 1 and a half years, an average of 10.9 blades can be grown and 16.4 roots can be grown. Taking the number of leaves greater than 9.4 and the number of roots greater than 12.1 at the same time as the strong seedling index, the seedling growth rate of Clivia seeds treated with this treatment is about 90% after 18 months of cultivation, and they also show strong growth potential, short and strong plants, developed root system, The characteristics of strong seedlings with plump and bright leaves.

[0039] The main data results of the above-mentioned ...

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Abstract

The invention discloses a method for strengthening clivia seedlings by utilizing ethyl methane sulfonate. The method includes before sowing clivia seeds, adopting an ethyl methane sulfonate solution of 0.15-0.2% (v / v) in concentration to soak the seeds; washing away ethyl methane sulfonate on the seeds after soaking, and soaking; after sowing the clivia seeds after being treated, performing separate planting when the clivia seedlings grow to 1.2-1.8cm in height; performing field planting when the clivia seedlings grow to be with 4-5 leaves after separate planting. In the method, after the clivia seeds after being treated by ethyl methane sulfonate (EMS) are sown, visible variation is not increased, seedling emergence rate is similar to that of a material which is not treated, and seedling strengthening rate of clivia can be increased effectively. Although the seeds after being treated grow more slowly at the beginning stage of sowing, the seeds gradually grow quickly after 12 mouths and show strong seedling features like high growing vigor, stocky plant, strong root system and fleshy and bright leaf.

Description

technical field [0001] The invention belongs to the technical field of Clivia plant cultivation, and in particular relates to a method for making Clivia seedlings strong by using ethyl methanesulfonate. Background technique [0002] Ethyl methylsulfonate, the English name is Ethylmethylsulfone (abbreviated as EMS, hereinafter EMS is used to represent ethyl methylsulfonate), the molecular formula is CH 3 SO 2 OC 2 h 5 , is a point mutation chemical mutagen commonly used in the creation of new germplasm and mutant libraries. In recent years, it has been found that EMS not only has mutagenic effects, but also has plant growth regulation effects. Zhang Bingxue, Wen Xiaopeng's research shows, with low-concentration EMS short-term soaking dragon fruit seeds, can improve seed germination speed and uniformity (EMS is to the influence of dragon fruit seed germination, Mountain Agricultural Biology Acta, 2013 (3): 194~197). It has been reported in the literature that treatment o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00A01G1/00
Inventor 张建军周音陈敏敏
Owner SHANGHAI ACAD OF AGRI SCI
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