Molecularly specific labeling primers of sakura variety 'Hirano Moseu' and detection method thereof

Abstract

The invention relates to a pair of molecularly specific labeling primers of a sakura variety 'Hirano Moseu' having high specificity and a method capable of rapidly identifying the sakura variety 'Hirano Moseu'. The sequences of the primers are as follows: a forward primer: 5'-AGCGGCCGCATATGGATTGTTACC-3' and a reverse primer: 5'-GGCCGCATCGGGCGAAGA-3'. By the molecularly specific labeling primers, the sakura variety 'Hirano Moseu' can be rapidly and early identified and the method is simple, fast and accurate and is an irreplaceable molecular means for apparently characterizing and identifying the sakura variety.

Description

(1) Technical field [0001] The invention relates to a molecular specific marker primer and an identification method of the cherry blossom variety "Pingye Meibei". (2) Background technology [0002] Cherry blossoms belong to the genus Cerasus of the Rosaceae (Rosaceae) and are world-renowned ornamental plants. At present, there are more than 300 known varieties of cherry blossoms, which have great application value in gardens because of their beautiful plant shape, bright colors and long flowering period. In recent years, cherry blossoms have been planted on a large scale in various parts of my country and used in parks, schools, streets, courtyards and other green spaces, becoming one of the main ornamental flowers and trees in early spring. Beijing, Wuhan, Qingdao, Shanghai and other cities hold cherry blossom festivals every year, and the cherry blossom industry has developed rapidly. This is of great significance to building a beautiful China, increasing farmers' income,...

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Problems solved by technology

After long-term investigation work, we found that 20 late cherry varieties include Hongli (Cerasus serrulata'Benigasa'), Honghua (C.serrulata'Kouka'), Yiye (C.serrulata'Hisakura'), Fulushou ( C.serrulata'Contorta'), Samantabhadra (C.serrulata'Albo-rosea'), Songyue (C.serrulata'Superba'), Yujin (C.serrulata'Grandifora'), Hongfeng (Cerasus×sieboldii' Beni‐yutaka'), Royal Clothes Yellow (C.serrulata'Gioiko'), Guanshan (C.serrulata'Kanzan'), Lantern (C.serrulata'Ojochin'), Eightfold Red Branch Weeping (C.spachiana'Plena Rosea' ), Ichihara Huwei (C.serrulata'Albo Plena'), Sakuyahime (Cerasus×yedoensis'Sakuyahime'), Suzaku (C.serrulata'Shujaku'), Yang Guifei (C.serrulata'Mollis'), Yuqing Zhiwei (C.spachiana'Ujou‐shidare'), Chrysanthemum weeping cherry (C.serrulata'Plena-pendula'), Hirano sister back (C.serrulata'Imose'), Asahiyama cherry (C.serrulata'Asahiyama') in the garden It is widely used, but the phenomenon of "same names and different objects" and "same objects and different names" of these varieties is also very serious, and it is difficult to identify them from the aspect of phenotypic characteristics, which brings many difficulties to the application, promotion and selection of new varieties of garden plants. Therefore, Focusing on the development of stable and specific DNA fingerprints for these varieties at the molecular level is the scientific way to achieve accurate and rapid identification of cherry blossom varieties

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