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Efficient phosphorus-solubilizing aspergillus niger strain and application thereof

A technology of Aspergillus niger and phosphate-solubilizing bacteria, which is applied in the field of highly efficient phosphorus-solubilizing Aspergillus niger strains, can solve the problems of low grade, increased agricultural production costs, and large energy consumption, so as to increase the content of available phosphorus, achieve remarkable phosphorus-solubilizing effect, The effect of increasing yield

Inactive Publication Date: 2015-05-27
QINGDAO VLAND BIOTECH GRP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] From the perspective of sustainable agricultural development, there are certain problems in the first way. First, phosphorus is a non-renewable resource. According to the proven reserves and mining speed of phosphorus rock, the existing phosphorus rock resources in the world can only last for 100 years. In addition,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1 Preliminary screening of microorganisms in soil

[0019] Preparation of soil diluent: take calcareous soil from the mountainous area of ​​southern Jinan, weigh 0.5g soil sample and dissolve it in 4.5ml sterile water to make a 1:10 soil solution, then draw 0.5ml soil solution and place it in 4.5ml without In the bacteria water, make a 1:100 soil solution, and by analogy with this method, prepare 1:10 6 -10 7 Dilute the soil solution.

[0020] Take 0.1ml of the soil dilution solution and evenly spread it on the insoluble inorganic phosphorus solid medium, and invert it in a 30℃ incubator for 2-3 days. Observe the colonies grown on the medium. Among them, 11 colonies have obvious surrounding The color changes and there are transparent circles, which are named YW-1, YW-2, YW-3,..., YW-11.

Example Embodiment

[0021] Example 2 Re-screening of Phosphate Solubilizing Microorganisms

[0022] The 11 strains obtained from the preliminary screening in Example 1 were respectively inoculated on a poorly soluble inorganic phosphorus solid medium and cultured at 30°C for 3 days. The size of the transparent circle around the colony was observed. It was found that the five bacteria with the largest transparent circle were YW- 2. YW-3, YW-7, YW-9, YW-10.

[0023] Inoculate the five bacteria with the largest transparent circle into 50 mL of insoluble inorganic phosphorus liquid medium, culture at 30°C, 200 rpm, for 6 days, and use the liquid phosphorus solubilizing medium without any bacteria as the control group; test and culture separately The number of viable bacteria in the liquid, the specific results are shown in Table 1.

[0024] 2.1 Plotting of phosphorus standard curve

[0025] Take 5mg / l of phosphorus standard solution 0.0, 0.2, 0.4, 0.8, 1.6, 2.0, 3.2, 4.0ml in the test tube in turn, the...

Example Embodiment

[0031] Example 3 Identification of YW-9 strain

[0032] 1) Colony morphology of strain YW-9:

[0033] The colony is dark brown, thick and velvety, with round and neat edges; the back of the colony is colorless or the center is slightly yellowish brown. There are a large number of conidia on the front of the colony, brown and black, without exudate; the flora is dark brown, with developed hyphae, and the conidia head is like a "chrysanthemum", with two layers of spore small stems, and the conidia are spherical. It is black, dark brown.

[0034] 2) Molecular biological identification of YW-9 strain:

[0035] The YW-9 strain obtained by the above screening was identified by molecular biology method, and its 18s rDNA sequence was measured, and the blast comparison was performed in the GenBank nucleic acid database. Based on the colony morphology of the YW-9 strain and the 18srDNA comparison result, the applicant confirmed that the YW-9 strain is Aspergillus niger (Aspergillus niger ),...

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Abstract

The invention relates to the technical field of functional microorganism screening, and in particular provides an aspergillus niger strain having a phosphorus-solubilizing function; the aspergillus niger strain is preserved with preservation number of CCTCC NO: M2015004. Through aspergillus niger, insoluble phosphorus (Ca3(PO4)2) in an insoluble inorganic phosphorus liquid medium can be decomposed into soluble available phosphorus, so that the content of available phosphorus in the medium can reach up to 0.680mg/mL, and a phosphorus-solubilizing effect is significant. In rape field cultivation experiments, the aspergillus niger screened by the invention can effectively improve the content of available phosphorus in soil, so as to greatly increase the yield of planted crops and to achieve an unexpected technical effect. The aspergillus niger can be used for preparing phosphorus-solubilizing bacteria; and the aspergillus niger is widely applicable to agricultural production and is broad in prospect.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a high-efficiency phosphorus-solubilizing Aspergillus niger strain and application thereof. Background technique [0002] As one of the three major nutritional elements, phosphorus is not only a component of many organs, but also participates in many important life metabolic activities. Phosphorus plays an important role in the synthesis, decomposition and transportation of carbohydrates in crops, and can improve the utilization of sugar and starch. As well as the phosphorus nutrition of oil crops, the abundance of phosphorus in the soil has a particularly obvious impact on the yield and quality of crops. [0003] From the perspective of our country, 74% of the country's cultivated land is deficient in phosphorus. There are two ways to solve this problem: one is to increase the input of phosphorus sources, and the other is to increase the utilization ra...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/685
CPCC09K17/00C09K2101/00C12N1/145C12R2001/685
Inventor 苑伟伟唐波甄丽朱彩彩刘鲁民
Owner QINGDAO VLAND BIOTECH GRP
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