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A kind of Penicillium bailei and its application in agricultural production

A technology of Penicillium bailei and agricultural crops, applied to the field of Penicillium bailei and its application in agricultural production, can solve the problems of water body environmental safety threats, waste of phosphorus resources, etc. Effects of increased growth and reduced chlorophyll content

Active Publication Date: 2019-10-22
QINGDAO VLAND BIOTECH GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plants mainly use the available phosphorus in the soil, but more than 95% of the phosphorus in the soil is ineffective phosphorus that is difficult for plants to use. In order to meet the growth of crops, people often increase crop yield by applying a large amount of phosphorus fertilizer, which leads to the rapid loss of phosphate Accumulation in the soil not only causes a waste of phosphorus resources, but also poses a certain threat to the safety of the water environment

Method used

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  • A kind of Penicillium bailei and its application in agricultural production
  • A kind of Penicillium bailei and its application in agricultural production
  • A kind of Penicillium bailei and its application in agricultural production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Microbial Screening and Identification with Phosphorus Solubilizing Function

[0019] 1. Primary screening

[0020] Soil sample: soil taken from the Pengjia Phosphate Mine Area in Laizhou City, Shandong Province;

[0021] Preparation of soil dilution: Weigh 0.5g soil sample and dissolve it in 4.5ml sterile water to make a 1:10 soil solution, then draw 0.5ml soil solution from it and place it in 4.5ml sterile water to make a 1:100 soil solution Solution, by analogy with this method, prepare 1:10 6 -10 7 soil dilution solution.

[0022] Take 0.1ml of soil dilution solution and apply it evenly on the insoluble inorganic phosphorus solid medium (glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 0 0.3g, FeSO 4 .7H 2 0 0.03g, MnSO 4 .4H 2 0 0.03g, Ca 3 (PO 4 ) 2 5.0g, distilled water 1000ml, pH 7.0-7.5, agar 20g, sterilized at 115°C for 30min), cultured upside down in a 30°C incubator for 2-3 days, observe the colonies growing on t...

Embodiment 2

[0036] Example 2 Application of Penicillium bailei VLD-11 in Rapeseed Field Cultivation Experiment

[0037] 1. Preparation of Penicillium bayley VLD-11 bacterial liquid:

[0038] First, inoculate the above-mentioned Penicillium bailei VLD-11 on the PDA solid medium, culture at 30°C for 3-4 days until the spores mature, wash the grown spores with an appropriate amount of sterile saline, and transfer them to the potato liquid medium , mixed well and cultured at 30°C for 4 days, the spore content in the fermentation liquid was 10 9 -10 10 CFU / mL.

[0039] 2. Experimental location: Rapeseed greenhouse in Kutao Village, Laoshan District, Qingdao City. The overall soil condition is relatively uniform.

[0040] 3. Experimental process:

[0041] A square area of ​​2m × 2m was selected as an experimental area, and a total of 30 experimental areas were set up, and a distance of 1 meter was maintained between each experimental area.

[0042] There are 3 groups in the experiment: ①Bl...

Embodiment 3

[0048] Example 3 Application of Penicillium bailei VLD-11 in rice seedling cultivation

[0049] 1. Experimental location: No. 4 nursery greenhouse of the second team of Baoquanling Farm, Hegang City, Heilongjiang Province.

[0050] 2. Experimental treatment group design:

[0051] Blank control group: only adopt seedling raising substrate (purchased from Zhejiang Jinhai seedling raising substrate factory);

[0052] Bailey Penicillium VLD-11 treatment group: 1.2kg Bailey Penicillium VLD-11 spore powder (10 9 CFU / g) was mixed evenly with 1 ton of seedling-raising substrate; the managements such as sowing, irrigation and fertilization of the above two treatment groups were the same, and the whole seedling-raising period was about 40 days.

[0053] 3. Experimental results and analysis

[0054] (1) Evaluation of rice seedling morphology and chlorophyll content

[0055] 100 seedlings were randomly selected from the center of the seedling trays of each treatment group, rinsed with...

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Abstract

The invention relates to the technical field of functional microorganism screening, and specifically provides a strain of Penicillium bayley with a preservation number of CCTCC NO: M2015690. Described Penicillium bailei has remarkable phosphorus-dissolving effect, in field cultivation experiment of rapeseed, the average fresh weight and dry weight of the rapeseed of the Penicillium bailei treatment group increased by 354.7% and 297.8% respectively than the blank control group, and the available phosphorus content was higher than that of the blank group. The treatment group improved by 89.8%. The Penicillium bailei can also significantly improve the quality of rice seedlings, and can work synergistically with hydroxyapatite to effectively promote the fixation of free heavy metals, reduce the content of free heavy metals in the soil, and restore heavy metal-contaminated soil to a certain extent. bright future.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a penicillium bayley and its application in agricultural production. Background technique [0002] Phosphorus is one of the necessary nutrients for plant growth. The importance of phosphorus to plants is self-evident. At the same time, phosphorus is a component of various compounds in plants and participates in the metabolism of plants. It can not only increase crop content, but also Increase plant stress resistance. Plants mainly use the available phosphorus in the soil, but more than 95% of the phosphorus in the soil is ineffective phosphorus that is difficult for plants to use. In order to meet the growth of crops, people often increase crop yield by applying a large amount of phosphorus fertilizer, which leads to the rapid loss of phosphate The accumulation in the soil not only causes a waste of phosphorus resources, but also poses a certain threat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/20A01N63/04A01P21/00C09K17/40A01G22/22C12R1/80C12R1/125C12R1/07C12R1/11C12R1/145C12R1/685
CPCA01G22/00A01N63/30C09K17/40C12N1/14C12N1/20C12N1/145C12R2001/80
Inventor 宗睿李佩佩刘鲁民周茜
Owner QINGDAO VLAND BIOTECH GRP
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