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Pantoea dispersa and applications thereof

A technology for dispersing Pantoea and bacterial strains, which is applied in the direction of bacteria, microorganisms, and methods based on microorganisms, which can solve the problems of unable to release soil active phosphorus, affect crop growth, and lack of phosphorus, so as to improve plant growth and lower product prices , The effect of simple production process

Active Publication Date: 2014-11-05
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem to be solved: The red soil in southern China cannot be released due to the long-term combination of soil active phosphorus, which leads to the extreme shortage of phosphorus and affects the growth of crops in this area

Method used

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  • Pantoea dispersa and applications thereof
  • Pantoea dispersa and applications thereof
  • Pantoea dispersa and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Pantoea dispersa (Pantoea dispersa) cav.cy1CGMCC No.9359 was isolated from the root tissue of cassava (Manihot esculenta Crantz) planted in the red soil ecological experiment station. The specific method is as follows: randomly select cassava roots, and rinse the surface with deionized water. Tissues were cut into 10mm x 5mm pieces. Under the aseptic operating environment, soak the small pieces of tissue in 75vt.% ethanol for 1 minute, then transfer the small pieces to 5wt.% sodium hypochlorite solution and soak for 10 minutes to thoroughly sterilize the tissue surface. Take out the sterilized small piece and rinse it 8 times with sterile water. In order to confirm whether the tissue surface is thoroughly sterilized, touch each side of the small tissue piece twice on the sterilized LB solid medium plate, and then transfer it to the improved NBRIP solid medium, and culture it statically at 37°C for 5 sky. When plaques appeared on the medium, carefully pick them up wit...

Embodiment 2

[0031] The strains of Pantoea dispersa (Pantoea dispersa) cav.cy1CGMCC No.9359 were taken out from the refrigerator at 4°C, inoculated on LB solid slant medium, cultured at 36°C for 2 days, and activated. Under sterile conditions, pour sterile water and shake for 30s, then transfer the bacterial suspension to the improved NBRIP liquid medium, pH 6.8-8.0, 30°C, 160rpm shaker for 3 days. The fermented bacterial agent passed the pot experiment, applied 400mL diluted bacterial agent (10 7 CFUs mL -1 ) cultured for 25 days, the soluble phosphorus content in the red soil of the adding bacterial agent group was 2.1 times that of the control group ( image 3 ). Use a pressure cooker to sterilize the mixture of soybean flour and peanut straw (mass ratio 1:10) at high temperature, and fill the tank under a sterile environment. Inoculate the amount of bacterial liquid of the proliferated bacterial agent according to the ratio of 5% of the mass of the filled tank, stir and mix well, an...

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Abstract

The invention discloses pantoea dispersa and applications thereof. The bacterium strain has been preserved in a preservation unit authorized by the State Intellectual Property Office of the P.R.C., the preservation date is June 18th 2014, the name of the preservation unit is China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.9359. Sterilized straws or other agricultural wastes suitable for being taken as the carbon source and nitrogen source are subjected to solid fermentation so as to obtain a solid microbial agent, which can be directly applied in soil. The experiment results have shown that the microbial agent can prominently improve the phosphor element absorption for plants like peanut, cassava, and the like; is capable of improving the plant growth conditions, and increases the yield.

Description

technical field [0001] The invention belongs to microorganisms and applications thereof, in particular to a dispersed pantoea and application thereof. Background technique [0002] my country's red soil is mainly distributed in tropical and subtropical regions, with a total area of ​​2.18 million km 2 , accounting for 21.8% of the country's land area, and the arable land area accounts for about 28% of the country's total area. However, due to the severe degradation of soil fertility, especially phosphorus, in this area, crop growth is slow and productivity declines. Although the total phosphorus content in red soil is rich, the available phosphorus content that can be absorbed by crops is extremely low (<0.1) because it is easily fixed and leached by metal salts. Therefore, the lack of phosphorus has become one of the key factors limiting the growth of crops in red soil areas. Although the application of chemical phosphorus fertilizers in the past has increased the con...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/01
Inventor 陈晏樊剑波何园球陈葵
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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