K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith
A technology of phosphorus-dissolving microorganisms and organic fertilizers, applied in the field of intensive agricultural production, can solve problems such as weakening the ability of sustainable agricultural development, waste of raw materials, loss of nutrient resources, soil-plant systems, etc., to improve the ability of colonization and function , increase available phosphorus, wide source effect
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[0026] (1) Acquisition of Phosphorus Solubilizing Bacteria
[0027] Select the well-grown plant rhizosphere from the calcareous phosphorus-deficient soil to collect soil and plant roots, and save the samples at low temperature (4-15°C) and bring them back to the laboratory; adopt a series method to utilize PVK-improved solid plate selective medium (glucose 10g) , tricalcium phosphate 5g, ammonium sulfate 0.5g, sodium chloride 0.2g, magnesium sulfate 0.1g, potassium chloride 0.2g, yeast extract 0.5g, manganese sulfate 0.002g, ferrous sulfate 0.002g, 0.4% bromophenol blue 6ml, agar 18g, distilled water 1000ml, pH natural) at 37.5°C for 36-48 hours to obtain a single colony of phosphorus-solubilizing bacteria, and select the strain with a larger ratio of phosphorus-dissolving circle to colony circle for further shaker screening; use NBRIP liquid medium (glucose 10g, tricalcium phosphate 5g, magnesium chloride 5g, magnesium sulfate 0.25g, potassium chloride 0.2g, ammonium sulfate ...
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