K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith

A technology of phosphorus-dissolving microorganisms and organic fertilizers, applied in the field of intensive agricultural production, can solve problems such as weakening the ability of sustainable agricultural development, waste of raw materials, loss of nutrient resources, soil-plant systems, etc., to improve the ability of colonization and function , increase available phosphorus, wide source effect

Active Publication Date: 2009-12-16
PENYAO ENVIRONMENTAL PROTECTION
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  • Claims
  • Application Information

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Problems solved by technology

[0004] On the other hand, the solid organic waste in my country's agricultural product processing industry and the livestock and poultry manure after large-scale breeding not only seriously pollute the environment, but also greatly waste raw materials that can be used as organic fertilizers and microbial organic fertilizer products; a large amount of The loss of nutrient resources (C, N, P, K, S and trace elements) outside the soil-plant system obviously weakens the ability of my country's agricultural sustainable development

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  • K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith
  • K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith
  • K3 bacterial strain capable of dissolving calcium phosphate in soil and organic microbial fertilizer therewith

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Embodiment Construction

[0026] (1) Acquisition of Phosphorus Solubilizing Bacteria

[0027] Select the well-grown plant rhizosphere from the calcareous phosphorus-deficient soil to collect soil and plant roots, and save the samples at low temperature (4-15°C) and bring them back to the laboratory; adopt a series method to utilize PVK-improved solid plate selective medium (glucose 10g) , tricalcium phosphate 5g, ammonium sulfate 0.5g, sodium chloride 0.2g, magnesium sulfate 0.1g, potassium chloride 0.2g, yeast extract 0.5g, manganese sulfate 0.002g, ferrous sulfate 0.002g, 0.4% bromophenol blue 6ml, agar 18g, distilled water 1000ml, pH natural) at 37.5°C for 36-48 hours to obtain a single colony of phosphorus-solubilizing bacteria, and select the strain with a larger ratio of phosphorus-dissolving circle to colony circle for further shaker screening; use NBRIP liquid medium (glucose 10g, tricalcium phosphate 5g, magnesium chloride 5g, magnesium sulfate 0.25g, potassium chloride 0.2g, ammonium sulfate ...

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Abstract

The invention discloses a bacterial K3 strain capable of dissolving calcium phosphate in soil and the microbial organic fertilizer produced therefrom, belonging to the agricultural intensive production technology. A bacterium with a strong ability to dissolve calcium phosphate was isolated from calcareous soil. The bacterium was first produced by liquid fermentation, and then mixed with a specific organic carrier to make a phosphorus-dissolving microbial organic fertilizer. The fertilizer contained 5×107 / The number of phosphorus-solubilizing microorganisms above g, the total nitrogen content is 4% (more than 90% is organic nitrogen), the total potassium content is 1-2%, the total phosphorus content is 1-2%, and the organic matter content is 35%. The test results show that applying 100 kilograms of the microbial organic fertilizer per mu on calcareous soil can provide phosphorus nutrition to crops equivalent to applying 10 kilograms of P2O5 chemical phosphorus fertilizer per mu. The laboratory culture results show that the phosphorus bacteria can make the soluble phosphorus concentration in the calcium phosphate solution reach 643mg / L, which is more than 4 times higher than the latest foreign report.

Description

1. Technical field [0001] The invention discloses a bacterial strain K3 capable of dissolving calcium phosphate in soil and the microbial organic fertilizer produced therefrom, which belongs to the technical field of agricultural intensive production and is specially used for overcoming the phosphorus deficiency problem of crops on calcareous soil. 2. Background technology [0002] After the chemical phosphorus fertilizer is applied to the soil, the crops of the current season can only use about 15%, and about 85% of the phosphorus fertilizer is fixed in the soil and gradually ages and loses its effectiveness. Even the crops of the next season can hardly use the phosphorus fertilizer applied in the previous season. . Therefore, farmers have to apply chemical phosphate fertilizers every season. In this way, on the one hand, a large amount of phosphate rock resources are exhausted. It is predicted that the existing high-grade phosphate rock resources in my country will be comp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08C12R1/385
Inventor 沈其荣杨兴明徐阳春黄启为
Owner PENYAO ENVIRONMENTAL PROTECTION
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