Penicillium bilaji and application thereof in agricultural production

A technology of Penicillium bailey and crops, applied to Penicillium bailey and its application in agricultural production, can solve the problems of water environment safety threat, waste of phosphorus resources, etc. Effects of increased and decreased chlorophyll content in Changhe

Active Publication Date: 2017-05-31
QINGDAO VLAND BIOTECH GRP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plants mainly use the available phosphorus in the soil, but more than 95% of the phosphorus in the soil is ineffective phosphorus that is difficult for plants to use. In order to meet the growth of crops, people often increase crop yield by applying a large amount of phosphorus fertilizer, which leads to the rapid loss of phosphate Accumulation in the soil not only causes a waste of phosphorus resources, but also poses a certain threat to the safety of the water environment

Method used

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  • Penicillium bilaji and application thereof in agricultural production
  • Penicillium bilaji and application thereof in agricultural production
  • Penicillium bilaji and application thereof in agricultural production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Microbial Screening and Identification with Phosphorus Solubilizing Function

[0019] 1. Primary screening

[0020] Soil sample: soil taken from the Pengjia Phosphate Mine Area in Laizhou City, Shandong Province;

[0021] Preparation of soil dilution: Weigh 0.5g soil sample and dissolve it in 4.5ml sterile water to make a 1:10 soil solution, then draw 0.5ml soil solution from it and place it in 4.5ml sterile water to make a 1:100 soil solution Solution, by analogy with this method, prepare 1:10 6 -10 7 soil dilution solution.

[0022] Take 0.1ml of soil dilution solution and apply it evenly on the insoluble inorganic phosphorus solid medium (glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 0 0.3g, FeSO 4 .7H 2 0 0.03g, MnSO 4 .4H 2 0 0.03g, Ca 3 (PO 4 ) 2 5.0g, distilled water 1000ml, pH 7.0-7.5, agar 20g, sterilized at 115°C for 30min), cultured upside down in a 30°C incubator for 2-3 days, observe the colonies growing on t...

Embodiment 2

[0036] Example 2 Application of Penicillium bailei VLD-11 in Rapeseed Field Cultivation Experiment

[0037] 1. Preparation of Penicillium bayley VLD-11 bacterial liquid:

[0038] First, inoculate the above-mentioned Penicillium bailei VLD-11 on the PDA solid medium, culture at 30°C for 3-4 days until the spores mature, wash the grown spores with an appropriate amount of sterile saline, and transfer them to the potato liquid medium , mixed well and cultured at 30°C for 4 days, the spore content in the fermentation liquid was 10 9 -10 10 CFU / mL.

[0039] 2. Experimental location: Rapeseed greenhouse in Kutao Village, Laoshan District, Qingdao City. The overall soil condition is relatively uniform.

[0040] 3. Experimental process:

[0041] A square area of ​​2m × 2m was selected as an experimental area, and a total of 30 experimental areas were set up, and a distance of 1 meter was maintained between each experimental area.

[0042] There are 3 groups in the experiment: ①Bl...

Embodiment 3

[0048] Example 3 Application of Penicillium bailei VLD-11 in rice seedling cultivation

[0049] 1. Experimental location: No. 4 nursery greenhouse of the second team of Baoquanling Farm, Hegang City, Heilongjiang Province.

[0050] 2. Experimental treatment group design:

[0051] Blank control group: only adopt seedling raising substrate (purchased from Zhejiang Jinhai seedling raising substrate factory);

[0052] Bailey Penicillium VLD-11 treatment group: 1.2kg Bailey Penicillium VLD-11 spore powder (10 9 CFU / g) was mixed evenly with 1 ton of seedling-raising substrate; the managements such as sowing, irrigation and fertilization of the above two treatment groups were the same, and the whole seedling-raising period was about 40 days.

[0053] 3. Experimental results and analysis

[0054] (1) Evaluation of rice seedling morphology and chlorophyll content

[0055] 100 seedlings were randomly selected from the center of the seedling trays of each treatment group, rinsed with...

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Abstract

The invention relates to the technical field of function microbial screening and specifically provides a penicillium bilaji bacterial strain with a preservation number of CCTCC NO: M2015690. The penicillium bilaji is remarkable in phosphate-dissolving effect; in a rape field cultivation experiment, the average fresh weight and dry weight of rapes in a penicillium bilaji treatment group are separately increased by 354.7% and 297.8% in comparison with those of a blank control group, and the effective phosphor content is increased by 89.8% in comparison with that of the blank control group. The penicillium bilaji further can remarkably improve the seedling quality of rice, can cooperatively act with hydroxyapatite to effectively promote fixing of free-state heavy metal, so that the content of the free-state heavy metals in the soil is reduced, soil contaminated by the heavy metals is restored to a certain extent, and the prospect is wide.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a penicillium bayley and its application in agricultural production. Background technique [0002] Phosphorus is one of the necessary nutrients for plant growth. The importance of phosphorus to plants is self-evident. At the same time, phosphorus is a component of various compounds in plants and participates in the metabolism of plants. It can not only increase crop content, but also Increase plant stress resistance. Plants mainly use the available phosphorus in the soil, but more than 95% of the phosphorus in the soil is ineffective phosphorus that is difficult for plants to use. In order to meet the growth of crops, people often increase crop yield by applying a large amount of phosphorus fertilizer, which leads to the rapid loss of phosphate The accumulation in the soil not only causes a waste of phosphorus resources, but also poses a certain threat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20A01N63/04A01P21/00C09K17/40A01G1/00C12R1/80C12R1/125C12R1/07C12R1/11C12R1/145C12R1/685
CPCA01G22/00A01N63/30C09K17/40C12N1/14C12N1/20C12N1/145C12R2001/80
Inventor 宗睿李佩佩刘鲁民周茜
Owner QINGDAO VLAND BIOTECH GRP
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