Rapid diagnosis and detection test paper card for detecting swine fever as well as preparation and application method of rapid diagnosis and detection test paper card

Abstract

The invention discloses a rapid diagnosis and detection test paper card for detecting swine fever as well as a preparation and application method of the rapid diagnosis and detection test paper card. The rapid diagnosis and detection test paper card comprises a PVC base plate, a sample pad, a gold-labelled antibody glass fiber membrane, a nitrocellulose coating membrane, water absorption paper, a detection line and a quality control line. The rapid diagnosis and detection test paper card is applied based on a colloidal gold immunochromatography technology; in a detection process, a swine fever antigen in a sample is combined with a colloidal gold coated antibody to form an antigen-antibody complex, the antigen-antibody complex flows to the other end of an NC membrane along test paper, and when the complex flows to a detection line area on the membrane, a specific antibody fixed on the membrane can capture the complex and gradually coagulate the complex to form a visible detection line, an uncombined colloidal gold antibody flows through the detection line area and is captured by a second antibody of the quality control line to form a visible quality control line, and if the quality control line appears, the occurrence of immunochromatography can be determined, namely, the test paper is valid; and if the detection line appears, a swine fever virus can be determined to exist in the sample. The rapid diagnosis and detection test paper card disclosed by the invention can be used for judging results within 5 minutes, and has the characteristics of short diagnosis time and strong specificity.

Description

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AI Technical Summary

Benefits of technology

This patented quick diagnose testing device uses special materials called colloids (colloid particles) made from polysaccharides or proteins attached to silica gel. These tiny objects are mixed together during manufacturing process before being used in tests like ELISA. They flow downstream towards an NM membranes where they capture certain substances found inside them. If there were any suspicious material present at this location, these particles could stick out onto another part of the machine's sensors causing false alarms. Colonies containing these agents have been developed quickly enough to make sure their presence was detected early.

Problems solved by technology

This patented technical problem addressed in this patents relates to developing efficient ways to identify diseases like swine flu viral outbreaks through testing techniques based solely on laboratory analysis. Current technologies have limitations including slow sample preparations, expensive reagent costs, complicated procedures requiring specialized expertise, poor sensitivity, difficulty obtaining results due to variations between different animals being tested, and potential health hazards associated therewith.

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