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HPV/CYAA-based chimeric proteins and their uses in the induction of immune responses against HPV infection and HPV-induced disorders

A technology of chimeric protein and protein, applied in the field of chimeric protein, can solve the problems of weak immune response of antigen cells and unprotected cellular immune response

Inactive Publication Date: 2015-06-24
DZHENTISEL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This results in a weak or no protective cellular immune response against the antigen

Method used

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  • HPV/CYAA-based chimeric proteins and their uses in the induction of immune responses against HPV infection and HPV-induced disorders
  • HPV/CYAA-based chimeric proteins and their uses in the induction of immune responses against HPV infection and HPV-induced disorders
  • HPV/CYAA-based chimeric proteins and their uses in the induction of immune responses against HPV infection and HPV-induced disorders

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0352] I. Materials and methods

[0353] mouse

[0354] Six-week-old female C57BL / 6 mice (H-2 b ). Mice were housed under pathogen-free conditions with ad libitum access to water and food. Procedures involving animals and their care complied with Genticel guidelines, which adhere to national and international laws and policies, and were reviewed by local ethics committees.

[0355] For some of the HPV types used in the present invention, other mice with different genetic backgrounds can be advantageously used. Such mice can be purchased from Janvier Laboratories and include: DBA / 2JRi (H2K d / H2D d ), or CBA / JRi (H2K k / H2D k )SJL / JRi(H2K s / H2D s ) and FVB / NRi (H2K q / H2D q ).

[0356] Other mice, such as humanized mice, which have HLA, particularly the HLA-A2 haplotype, are also of interest in determining the immune response elicited by the constructs of the invention. Transgenic HLA-A2.1 mice can be purchased from TACONIC (USA) in mice expressing the human HLA...

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Abstract

The invention relates to a chimeric protein comprising or consisting of, from N-terminal to C-terminal, (a) a N-terminal part of a Bordetella CyaA protein (b) a heterologous polypeptide comprising antigens originating from different HPVs, and (c) a C-terminal part of a Bordetella CyaA protein. The invention also relates to a polynucleotide encoding this chimeric protein. A composition comprising at least one chimeric protein(s) of the invention and the prophylactic and / or therapeutic uses of said composition are also part of the invention.

Description

technical field [0001] The present invention relates to a chimeric protein comprising or consisting of: from N-terminus to C-terminus, (a) the N-terminal portion of the Bordetella CyaA protein; (b) a heterologous polypeptide, which Contains antigens from different HPVs; and (c) the C-terminal portion of the Bordetella CyaA protein. The present invention also relates to a polynucleotide encoding the chimeric protein. Another aspect of the present invention also relates to a composition comprising at least one chimeric protein of the present invention and the prophylactic and / or therapeutic use of said composition. Background technique [0002] The adenylyl cyclase (CyaA) of the Bordetella type, specifically Bordetella pertussis, has been extensively described as a recombinant vector that efficiently delivers polypeptides such as antigens to antigen presentation In the cytosol of the cell (APC) [1]、[2]、[3] . In addition, recombinant CyaAs have been used to effectively trea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295C07K14/235C07K14/025
CPCA61K39/12A61K2039/55561A61K2039/585A61K2039/70A61P31/04A61P31/12A61P35/00A61P37/04C07K14/005C07K14/235C07K2319/00C12N9/88C12N2710/20022C12N2710/20034C12Y406/01001A61K2039/6068A61P31/20C07K2319/10C12N7/00
Inventor Y·米斯里P·布里多诺A·古比耶
Owner DZHENTISEL
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