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Chlamydia antigen compositions and uses thereof

A composition, technology of the genus Chlamydia, applied in the direction of Chlamydia antigen components, drug combinations, active ingredients of nitro compounds, etc., can solve problems such as increased risk of HIV infection

Inactive Publication Date: 2015-07-29
THE UNIV OF BRITISH COLUMBIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, women infected with Chlamydia trachomatis have an increased risk of HIV infection after exposure

Method used

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  • Chlamydia antigen compositions and uses thereof
  • Chlamydia antigen compositions and uses thereof
  • Chlamydia antigen compositions and uses thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0153] Molecular cloning, expression and purification of recombinant fusion proteins

[0154] PmpE, pmpF, pmpG and ppmH DNA fragments were generated by PCR using genomic DNA isolated from C. trachomatis murine biotypes. The DNA fragment generated by PCR was cloned stepwise into the pET32a expression vector (GE Healthcare) after restriction enzyme digestion using standard molecular biology techniques. For all four pmp genes, only the region encoding the bearer domain of the Pmp protein was cloned into the expression vector. Fig. 1 shows the amino acid sequences of the PmpE, PmpF, PmpG and PmpH proteins of Chlamydia murine biotype. The portion of the bearer domain between amino acids 18-575, 20-575, 25-555 and 27-575 of the whole protein of PmpE, PmpF, PmpG and PmpH, respectively, was used. A C-terminal His-tag was introduced into all fusion proteins. The plasmid containing the pmp gene was transformed into E. coli strain BL21(DE3) (Strategene), wherein the lac promoter for e...

Embodiment 2

[0159] To evaluate protection against intragenital infection with Chlamydia trachomatis murine biotypes, C57, Balb / c or C3H mice were immunized with PmpE, F, G, H plus MOMP (single protein or fusion protein) in the following groups .

[0160] C57 mice: (1) PmpE+PmpF+PmpG+PmpH+MOMP (mixed); (2) PmpE-F fusion+PmpG-H fusion+MOMP (fusion); (3) PBS; (4) live EB .

[0161] Balb / c mice: (5) PmpE+PmpF+PmpG+PmpH+MOMP (mixed); (6) PmpE-F fusion+PmpG-H fusion+MOMP (fusion); (7) PBS; (8) live EB.

[0162] C3H mice: (9) PmpE+PmpF+PmpG+PmpH+MOMP (mixed); (10) PmpE-F fusion+PmpG-H fusion+MOMP (fusion); (11) PBS; (12) live EB .

[0163] Mice were immunized three times at 2-week intervals with the fusion protein formulated with DDA / MPL. PBS was used as a negative control, and mice infected once with 1,500 inclusion forming units (IFU) of live Chlamydia trachomatis murine (EB) intranasally were used as positive controls. Four weeks after the final immunization or 8 weeks after live Chlamy...

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Abstract

The present invention provides in part fusion proteins derived from Chlamydia spp. The present invention also provides in part methods for treating or preventing Chlamydia infection using the fusion proteins.

Description

technical field [0001] The present invention relates to bacterial infections. More specifically, the present invention provides, in part, a fusion protein for resisting Chlamydia infection. Background technique [0002] Chlamydia trachomatis is an intracellular pathogen that causes more than 92 million sexually transmitted infections and 85 million ocular infections per year worldwide (Starnbach, M.N., and N.R. Roan. 2008. Conquering sexually transmitted diseases. Nat Rev Immunol 8:313-317.). Sexually transmitted Chlamydia trachomatis is a major cause of medium and long-term disease sequelae (such as infertility and ectopic pregnancy) in women (Brunham, R.C, D.J. Zhang, X. Yang, and G.M. McClarty. 2000. The potential for vaccine development against chlamydial infection and disease. J Infect Dis 181 Suppl 3:S538-543; Igietseme, J.U., C.M. Black, and H.D. Caldwell. 2002. Chlamydia vaccines: strategies and status. BioDrugs 16:19-35). Chlamydia trachomatis infection in women ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/118A61K31/04A61P37/04
CPCA61K2039/543A61K39/118A61K2039/55555A61K2039/70A61P37/04
Inventor R·C·布鲁汉姆K·P·卡鲁纳卡拉恩俞红
Owner THE UNIV OF BRITISH COLUMBIA
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