A portable microfluidic organophosphorus pesticide detection device and method

Abstract

The invention discloses a portable microfluidic detection apparatus and method for organophosphorus pesticide. A microfluidic chip is fixed at the upper part in a shell of a detector; the microfluidic chip is provided with, from left to right, a sample pretreatment and introduction chamber, an impurity filtering assembly, a mixed driving assembly, a porous medium enzyme reaction assembly, a reaction chamber, a photoelectric detection chamber and a waste liquid discharge outlet which are successively connected and communicated; a permanent magnet fixedly connected with the upper surface of a waterproof rotary grinding plug is arranged under an electromagnet, and the waterproof rotary grinding plug is located over the sample pretreatment and introduction chamber and can move downwardly to match with the sample pretreatment and introduction chamber; a grinding motor is installed in the waterproof rotary grinding plug and is connected with a grinding rotary cake through a grinding motor shaft; and a 405-nm laser diode is fixedly arranged over a photoelectric detection chamber, and a photoelectric detector is fixedly arranged under the photoelectric detection chamber. The portable microfluidic detection apparatus and method provided by the invention have a sample pretreatment function and can improve sample purity, enhance a contact area between organophosphorus hydrolase and a detection fluid and effectively guarantee detection effects.

Description

Technical field [0001] The invention relates to the field of microfluidic and pesticide detection, in particular to a microfluidic organic phosphorus pesticide detection device and method. Background technique [0002] At present, pesticides, especially organophosphorus pesticides, are still the main measures for the prevention and control of crop diseases and insect pests. However, pesticides have the characteristics of high toxicity and long durability. The widespread use of pesticides has caused serious dietary safety problems. Therefore, how to implement pesticide detection in a portable, fast and accurate manner has become an important means to ensure food safety. [0003] Chinese patent application number: 201410092671.5, the microfluidic chip system disclosed under the name "A microfluidic chip system and method for on-site detection of pesticide residues" consists of a disposable microfluidic chip and a portable instrument, microfluidic The chip is composed of fluid inlet ...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a portable low-noise microfluidic organophosphorus pesticide detection device with simple structure, easy operation and high degree of automation, based on the principle of organophosphorus hydrolysis color reaction (i.e. organic Phosphorus pesticides will undergo a color reaction when they encounter organophosphate hydrolase), with automatic sample pretreatment and detection functions

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