Application of icaritin in preparation of anti-NK/T cell lymphoma drug
A technology for icariin and lymphoma, which is applied in the field of icariin in the field of preparing anti-NK/T cell lymphoma drugs, can solve the problem that the use of icariin has not been reported yet.
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Embodiment 1
[0029] Example 1: Icaritin Inhibits NK / T Cell Lymphoma Cell Proliferation and Induces Cell Apoptosis Test
[0030] 1. Materials and methods
[0031] 1. Cell lines, using cell lines commonly used in the study of NK / T cell lymphoma: the human NK / T cell lymphoma cell lines SNK-10 and SNT-8 in this example were purchased from Tokyo Medical and Dental University, Japan, both EB If the virus is positive, the cell line is treated with PRIM-1640 medium containing 10% inactivated human serum and 700U / ml IL-2 at 37°C with a volume fraction of 5% CO 2 , Routine culture under fully saturated humidity conditions, subculture once every 2 to 3 days, and use logarithmic growth phase cells in the experiment;
[0032] 2. Detection of cell viability
[0033] Adjust the cell suspension concentration of SNK-10 and SNT-8 cell lines to 1×10 5 / ml, added in 96-well culture plate, 100 μl per well, respectively added equal volumes of different concentrations of icariin (drug concentration used for S...
Embodiment 2
[0056] Icaritin induces the activation of EBV in SNK-10 cells and increases its sensitivity to GCV 1. Materials and methods
[0057] 1. Cell line: human NK / T cell lymphoma cell line SNK-10, the culture method is the same as above.
[0058] 2. The effect of icariin on the expression of EBV-related genes
[0059] Adjust the concentration of SNK-10 cell suspension to 4×10 5 / ml, the cells were inoculated in a 6-well plate, 2ml per well, and equal volumes of different concentrations of icariin (drug concentration as before) were added to culture for 48h, RNA was extracted, reverse-transcribed into cDNA, and then Real-timePCR (using Takara CompanySYBR PremixExTaqTM kit), to detect the expression of EBV lytic phase genes (BZLF1, BRLF1 and BMRF1) and latent phase gene EBNA1, using GAPDH as an internal reference, calculate the △Ct (Ct value of the target gene - GAPDH gene Ct value) of each sample, and take the lowest expression The sample is used as a calibration sample to calcula...
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