Application of icaritin in preparation of anti-NK/T cell lymphoma drug

A technology for icariin and lymphoma, which is applied in the field of icariin in the field of preparing anti-NK/T cell lymphoma drugs, can solve the problem that the use of icariin has not been reported yet.

Active Publication Date: 2015-11-25
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There is no report about the use of icariin in the preparation of anti-NK / T cell lymphoma drugs

Method used

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  • Application of icaritin in preparation of anti-NK/T cell lymphoma drug
  • Application of icaritin in preparation of anti-NK/T cell lymphoma drug
  • Application of icaritin in preparation of anti-NK/T cell lymphoma drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Icaritin Inhibits NK / T Cell Lymphoma Cell Proliferation and Induces Cell Apoptosis Test

[0030] 1. Materials and methods

[0031] 1. Cell lines, using cell lines commonly used in the study of NK / T cell lymphoma: the human NK / T cell lymphoma cell lines SNK-10 and SNT-8 in this example were purchased from Tokyo Medical and Dental University, Japan, both EB If the virus is positive, the cell line is treated with PRIM-1640 medium containing 10% inactivated human serum and 700U / ml IL-2 at 37°C with a volume fraction of 5% CO 2 , Routine culture under fully saturated humidity conditions, subculture once every 2 to 3 days, and use logarithmic growth phase cells in the experiment;

[0032] 2. Detection of cell viability

[0033] Adjust the cell suspension concentration of SNK-10 and SNT-8 cell lines to 1×10 5 / ml, added in 96-well culture plate, 100 μl per well, respectively added equal volumes of different concentrations of icariin (drug concentration used for S...

Embodiment 2

[0056] Icaritin induces the activation of EBV in SNK-10 cells and increases its sensitivity to GCV 1. Materials and methods

[0057] 1. Cell line: human NK / T cell lymphoma cell line SNK-10, the culture method is the same as above.

[0058] 2. The effect of icariin on the expression of EBV-related genes

[0059] Adjust the concentration of SNK-10 cell suspension to 4×10 5 / ml, the cells were inoculated in a 6-well plate, 2ml per well, and equal volumes of different concentrations of icariin (drug concentration as before) were added to culture for 48h, RNA was extracted, reverse-transcribed into cDNA, and then Real-timePCR (using Takara CompanySYBR PremixExTaqTM kit), to detect the expression of EBV lytic phase genes (BZLF1, BRLF1 and BMRF1) and latent phase gene EBNA1, using GAPDH as an internal reference, calculate the △Ct (Ct value of the target gene - GAPDH gene Ct value) of each sample, and take the lowest expression The sample is used as a calibration sample to calcula...

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Abstract

The invention belongs to the field of traditional Chinese medicine, and particularly relates to application of icaritin in preparation of anti-NK/T cell lymphoma drug. Experiments of intervening human NK/L cell lymphoma cell strains (SNK-10 and SNT-8) through icaritin confirm that icaritin in vitro can remarkably inhibit proliferation of SNK-10 and SNT-8 cells, obviously promote apoptosis of the SNK-10 and SNT-8 cells, increase endogenous Caspase protein, promote expression of apoptin Bax and inhibit expression of Bc1-2 and pBad, and can induce activation of EB viruses in the SNK-10 cells and improve sensitivity, to anti-virus drug, of tumor cells. Icaritin can serve as a pharmaceutically active ingredient to prepare the anti-NK/T cell lymphoma drug and can serve as a pharmaceutically acceptable carrier to prepare drug combinations resistant to NK/T cell lymphoma, and further, icaritin can be combined with the anti-virus drug to prepare anti-NK/T cell lymphoma sensitizing drug.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a new medicinal application of icariin, an active ingredient of traditional Chinese medicine, in particular to the application of icariin in preparing anti-NK / T cell lymphoma drugs. Background technique [0002] NK / T-cell lymphoma is a highly aggressive form of non-Hodgkin lymphoma that occurs primarily in tissues outside the lymph nodes. The early clinical manifestations of the disease are atypical, the course of the disease progresses rapidly, and the prognosis is poor. Currently, there are no specific chemotherapy drugs in clinical practice. Studies have shown that the onset of NK / T cell lymphoma is closely related to Epstein-Barr virus (EBV) infection, and EBV is a latent infection in tumor cells, so it brings difficulties to antiviral treatment. Some studies have induced EBV into lytic infection (activation) and combined antiviral drug ganciclovir (GCV) to target a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K31/522A61P35/00
Inventor 陈波斌吴婷王松梅吴金峰许小平汪萱怡
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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