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Children's gene evaluation and detection method

A detection method and technology for children, applied in the field of genetic evaluation and detection of children, can solve the problems of decreased dopamine activity, increased norepinephrine activity, affecting the function of the prefrontal lobe of the brain, etc.

Inactive Publication Date: 2015-11-25
GENE TARGET TECH
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Problems solved by technology

[0032] a.DBH: This gene encodes a oxidoreductase (DβHenzyme), which is responsible for converting dopamine into norepinephrine. Individuals with polymorphisms that promote the expression of this gene will convert a large amount of dopamine The formation of norepinephrine causes an imbalance of catecholamines in the body, and the activity of norepinephrine increases, while the activity of dopamine decreases, which in turn affects the function of the frontal lobe of the brain dominated by dopamine

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  • Children's gene evaluation and detection method

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Embodiment Construction

[0070] See figure 1 As shown, the genetic evaluation and detection method for children's fitness education of the present invention includes the following steps:

[0071] Step 1: Provide a nucleotide sample of a child; the nucleotide sample is DNA extracted from a child sample by nucleic acid purification technology. The sample can be blood or oral mucosal cells. The quality is evaluated by the ratio of absorbance value at the wavelength of 260nm / 280nm set by the spectrophotometer. In addition, the extracted DNA and the sample primer are subjected to a polymerization chain synthesis reaction. The polymerization chain synthesis reaction amplification method is used to amplify the subject’s Target gene nucleotide products, the PCR-synthesized products are then subjected to DNA analysis by sequencer;

[0072] Step 2: Identify the base characteristics of a single nucleotide polytype or multiple single nucleotide polytypes of the nucleotide sample; and

[0073] Step 3: When the individu...

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Abstract

The invention discloses a children's gene evaluation and detection method. The method is used for judging children adaptive education by detecting catechol-O-methyltransferase gene, synaptosome associated gene, brain-derived neurotrophic gene, hydroxytryptamine receptor gene, short memory regulation gene, muscarinic acetylcholine receptor gene, morphine receptor gene, developmental reading dyslexia susceptibility gene, dyslexia susceptibility candidate gene, fork head box P2 gene, contactin-associated protein gene, neuregulin gene, synaptosome associated gene and dopamine beta hydroxylase gene. Individual adaptability development performance degree is judged or evaluated by identifying whether single nucleotide polymorphism in nucleotide samples of children individuals is associated with the above genes or not to serve as application evaluation data in acquired learning.

Description

Technical field [0001] The invention relates to a method for genetic evaluation and detection of children, in particular to a method for detecting catechol-O-methyltransferase gene (COMT), synaptosome-associated gene (SNAP25), and brain-derived neurotrophic gene (BDNF), serotonin receptor gene (5HT2A), short-term memory regulation gene (KIBRA), muscarinic acetylcholine receptor gene (CHRM2), morphine receptor gene (OPRM1), developmental dyslexia susceptibility gene (KIAA0319) , Dyslexia susceptibility candidate gene (DYX1C1), forkhead box P2 gene (FOXP2), contact protein associated protein gene (CNTNAP2), neuregulin gene (NRG1), synaptosome associated gene (SEC9), dopamine β hydroxyl A genetic evaluation and detection method for children’s fitness education based on the single-nucleotide polymorphic characteristics of the DBH gene. Background technique [0002] Currently known children’s talent performance includes intelligence performance, memory, comprehension, learning abilit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈怡芳熊思恺
Owner GENE TARGET TECH
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