Method for determining microorganism varieties and pathogenic microorganism pollution conditions in air

A technology of microorganism species, air microorganisms, applied in the field of biology

Inactive Publication Date: 2015-11-25
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by this invention is how to dete

Method used

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  • Method for determining microorganism varieties and pathogenic microorganism pollution conditions in air
  • Method for determining microorganism varieties and pathogenic microorganism pollution conditions in air
  • Method for determining microorganism varieties and pathogenic microorganism pollution conditions in air

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Experimental program
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Effect test

Embodiment 1

[0108] Embodiment 1, the determination of microbial species and air pollution status in the air

[0109] 1. Determination of the types of microorganisms in the air

[0110] 1) Determination of microbial species in indoor air

[0111] When determining the types of microorganisms in the indoor air of a hospital, a total of 5 different indoor locations of the hospital (outpatient hall, two different wards, pharmacies, and charging offices) were selected for sample collection and determination of indoor microbial types. The specific methods are as follows :

[0112] 1. Sampling

[0113] Equipment: SKC air sampling pump; Pall detachable filter and PTEE filter membrane, the diameter of the PTEE filter membrane is matched with the detachable filter, and the pore size is 0.2 microns.

[0114] Methods: Put the SKC air sampling pump in a certain hospital room, adjust the intake speed of the SKC air sampling pump to 4L / min; put the detachable filter of Pall company with PTEE filter me...

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Abstract

The invention discloses a method for determining microorganism varieties and pathogenic microorganism pollution conditions in air. The method for determining microorganism varieties in air comprises the following steps: 1) acquiring microorganisms in air to be detected by using a sampling filter membrane to obtain microorganisms in the air to be detected on the sampling filter membrane; 2) extracting DNAs (deoxyribonucleic acids) in the air to be detected on the sampling filter membrane to obtain unpurified microorganism DNAs; purifying the unpurified microorganism DNAs with magnetic beads to obtain microorganism DNAs in the air; and 3) sequencing the microorganism DNAs in the air, and determining the microorganism varieties in the air to be detected according to the sequences of the microorganism DNAs in the air. The experiment proves that the method for determining microorganism varieties in air can be used for determining the microorganism varieties in the air, and the method for determining pathogenic microorganism pollution conditions in air can be used for determining the existence of pathogenic microorganism pollution in the air.

Description

technical field [0001] The invention relates to a method for determining the types of microorganisms in the air and the pollution status of pathogenic microorganisms in the field of biotechnology. Background technique [0002] The particle size range of suspended particles in the air is usually between 10nm and 100μm. Due to the irregular shape of atmospheric particulate matter, according to the size of aerodynamic diameter, atmospheric particulate matter is usually divided into TSP (Total suspended particulate, aerodynamic diameter ≤ 100 μm), PM10 (coarse particulate matter, aerodynamic diameter ≤ 10 μm), PM2 .5 (fine particulate matter, aerodynamic diameter ≤ 2.5 μm) and PM0.1 (ultrafine particulate matter, aerodynamic diameter ≤ 0.1 μm), etc. The international community’s widespread attention to PM10 and PM2.5 particles in the two particle size ranges is mainly due to two reasons: First, from the perspective of the particle size distribution range of suspended particles,...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/6869C12Q2535/122Y02A50/30
Inventor 朱听蒋靖坤田埂
Owner TSINGHUA UNIV
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