Method for determining microorganism varieties and pathogenic microorganism pollution conditions in air

A technology of microorganism species, air microorganisms, applied in the field of biology
CN105087797AInactive Publication Date: 2015-11-25TSINGHUA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TSINGHUA UNIV
Publication Date
2015-11-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for determining microorganism varieties and pathogenic microorganism pollution conditions in air. The method for determining microorganism varieties in air comprises the following steps: 1) acquiring microorganisms in air to be detected by using a sampling filter membrane to obtain microorganisms in the air to be detected on the sampling filter membrane; 2) extracting DNAs (deoxyribonucleic acids) in the air to be detected on the sampling filter membrane to obtain unpurified microorganism DNAs; purifying the unpurified microorganism DNAs with magnetic beads to obtain microorganism DNAs in the air; and 3) sequencing the microorganism DNAs in the air, and determining the microorganism varieties in the air to be detected according to the sequences of the microorganism DNAs in the air. The experiment proves that the method for determining microorganism varieties in air can be used for determining the microorganism varieties in the air, and the method for determining pathogenic microorganism pollution conditions in air can be used for determining the existence of pathogenic microorganism pollution in the air.
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Description

technical field

[0001] The invention relates to a method for determining the types of microorganisms in the air and the pollution status of pathogenic microorganisms in the field of biotechnology. Background technique

[0002] The particle size range of suspended particles in the air is usually between 10nm and 100μm. Due to the irregular shape of atmospheric particulate matter, according to the size of aerodynamic diameter, atmospheric particulate matter is usually divided into TSP (Total suspended particulate, aerodynamic diameter ≤ 100 μm), PM10 (coarse particulate matter, aerodynamic diameter ≤ 10 μm), PM2 .5 (fine particulate matter, aerodynamic diameter ≤ 2.5 μm) and PM0.1 (ultrafine particulate matter, aerodynamic diameter ≤ 0.1 μm), etc. The international community’s widespread attention to PM10 and PM2.5 particles in the two particle size ranges is mainly due to two reasons: First, from the perspective of the particle size distribution range of suspended particles,...

Claims

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