A kind of assay method of algal biomass in biological soil crust

A technology of biological soil and measurement method, which is applied to the measurement of algal biomass in biological soil crusts and the field of measurement of algal crust biomass, which can solve the inability to compare the relationship between algal crusts and the inability to accurately evaluate the contribution of algal crust biomass to achieve the effects of easy realization of the cultivation environment, elimination of weight interference, and assurance of accuracy

Active Publication Date: 2017-10-27
NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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Problems solved by technology

However, it is difficult to directly weigh the biomass of biological soil crusts and soil due to their close cementation. Therefore, it is impossible to compare the relationship between algal crusts and vascular plant biomass, and it is also impossible to accurately evaluate the impact of algal crusts biomass on Contribution rate of ecosystem productivity

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  • A kind of assay method of algal biomass in biological soil crust

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example 1

[0023] Example 1: Determination of Algal Crust Biomass Developed in Interdune Lowlands

[0024] In August 2014, 10 samples of well-developed algae crusts were collected from the lowland between dunes in the artificial vegetation sand-fixation area established in 1990 at the Shapotou National Station of the Chinese Academy of Sciences in Zhongwei City, Ningxia Hui Autonomous Region, and the area of ​​each sample was 9 cm 2 . Sampling uses a self-made 3 cm * 3 cm * 0.5 cm square stainless steel frame; specific methods, such as: algae crust sample sampling, sieving, cleaning, algal culture, determination of chlorophyll a and b, standard curve drawing and natural development The determination of algae biomass is as follows:

[0025] a. When sampling, first press the sampling frame firmly into the soil until the sampling frame is completely inserted into the soil, then gently take out about 50 cm inside the stainless steel frame with a shovel sterilized with 75% alcohol 2 Algae c...

example 2

[0032] Example 2: Determination of Algal Crust Biomass Developed on Sand Dune Tops

[0033] In August 2014, 10 samples of well-developed algae crusts were collected from the lowlands on the top of sand dunes in the artificial vegetation sand-fixation area established in 1990 at the Shapotou National Station of the Chinese Academy of Sciences in Zhongwei City, Ningxia Hui Autonomous Region. The area of ​​each sample was 9 cm2. A self-made square stainless steel frame of 3 cm * 3 cm * 0.5 cm is used for sampling; specific methods, such as sampling, sieving, cleaning, and culturing of algae crust samples are the same as in Example 1.

[0034] Determination of chlorophyll a and b: the extraction of chlorophyll adopts the mixed liquid method (extraction liquid: absolute ethanol: acetone: water =4.5:4.5:1). Put the collected algae crust samples into test tubes containing 10 mL of extract solution, plug them with rubber stoppers, and repeat each treatment three times; put them in a t...

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Abstract

The invention discloses a method for measuring algal biomass in biological soil crusts, and relates to the field of biology. The method comprises the steps of algae crust sample sampling, sieving, cleaning, algae cultivation, determination of chlorophyll a and b, standard curve drawing and algae biomass (natural growth) determination. Among them, the algae culture is cultivated with BG11 medium, and the algae used are mixed algae; the determination of chlorophyll a and b adopts the mixed solution method to extract chlorophyll (extraction solution: absolute ethanol: acetone: water = 4.5:4.5:1); standard curve The relationship between the total amount of chlorophyll a and b and algae biomass was regressed; the determination of the biomass of naturally growing algae was converted by the standard curve between the total amount of chlorophyll a and b and the biomass of cultured algae. The invention realizes the direct comparison between the algae crust and the vascular plant biomass, and provides a reliable way for more accurate calculation of the algae crust productivity and its function in the ecosystem.

Description

technical field [0001] The invention relates to a method for measuring the biomass of algae in biological soil crusts, which is suitable for measuring the biomass of algae crusts, and aims at realizing the comparison between the biomass of algae crusts and vascular plants, belonging to the field of biology. Background technique [0002] Biological soil crusts are the main builders and ecosystem engineers of desert ecosystems, and are an important feature of desert ecosystem composition and surface landscape. They are tiny particles on the soil surface that are surrounded by bacteria, microscopic fungi, cyanobacteria, diatoms or green algae, lichens and bryophytes that live in the shallow soil (or sometimes live on the soil surface), even up to a few millimeters above the soil surface, As well as non-vascular plant components commonly found in many landscapes, they use mycelium, rhizoids and exudates to "cement" to form complex and relatively stable aggregate structures with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 赵洋李新荣贾荣亮
Owner NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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