A Simple Isolation and Preservation Method of Aspergillus Oryzae

Abstract

The invention relates to the technical field of aspergillus oryzae isolation and preservation, in particular to a simple method for isolating and preserving aspergillus oryzae. Its separation method comprises the following steps: collect fresh yellow, green and black rice bent ball samples from diseased paddy fields, and dry at room temperature for 7-10d for subsequent use; select chlamydospore powder from the collected samples to cover rice bent rice with a thickness greater than 1mm Put the ball on the ultra-clean workbench and sterilize it with ultraviolet light for 30-40 minutes; take a single rice ball with tweezers under sterile conditions, and gently shake off a small amount of chlamydospore powder on the surface of the rice ball and put it on the PSA medium On; use a pipette gun to take 150-200ul sterile water drops on the PSA plate containing chlamydospores and so on. The method of the present invention can be used to quickly isolate and store the target bacterial strain, which solves the problems of time-consuming and low efficiency in the isolation of Aspergillus oryzae, and also solves the problems of pathogenicity degradation and space consumption of the bacterial strain during storage. .

Description

technical field

[0001] The invention relates to the technical field of aspergillus oryzae isolation and preservation, in particular to a simple method for isolating and preserving aspergillus oryzae. Background technique

[0002] Rice false smut is caused by Aspergillus oryzae ( Villosiclavavirens (Nakata) E.Tanaka & C. Tanaka) is an important fungal disease caused by the fungus infecting the panicle and forming a rice ball several times larger than the grain, and its surface is covered with a large amount of powdery yellow or dark green thick Cystospores. Rice curlers not only seriously threaten the yield and quality of rice, but also produce mycotoxins that are highly toxic to humans and livestock (Koiso et al., 1994; Li et al., 1995). In recent years, with the increase in the use of N fertilizer in rice and the promotion of hybrid rice in a large area, the severity of rice false smut is increasing. In severe years, the resulting yield loss can be as high as 50%. [00...