Simple method for separating and preserving ustilaginoidea virens

Abstract

The invention relates to the technical field of separating and preserving ustilaginoidea virens, in particular to a simple method for separating and preserving the ustilaginoidea virens. The separation method comprises the following steps that fresh yellow, green and black rice false smut ball samples are collected from an attacked rice field, drying is conducted at the room temperature for 7-10 d for standby application; rice false smut balls of which the covering depth of chlamydospore powder is larger than 1 mm are selected in the collected samples and placed on an ultra-clean working table, and sterilization is conducted through an ultraviolet lamp for 30-40 min; single-grain rice false smut balls are taken through tweezers under the aseptic condition, and a small amount of chlamydospore powder on the surfaces of the rice false smut balls is shaken off slightly on a PSA culture medium; 150-200 microliters of sterile water is taken through a pipette and drips on a PSA flat plate containing chlamydospore. By means of the simple method for separating and preserving the ustilaginoidea virens, the ustilaginoidea virens can be separated to the objective strains rapidly and preserved, the problems that time is consumed, and the efficiency is low during separation of the ustilaginoidea virens are solved, and meanwhile the problems that in the preservation process of the strains, the pathogenicity is deteriorated, and the space is occupied and consumed are solved.

Description

technical field [0001] The invention relates to the technical field of aspergillus oryzae isolation and preservation, in particular to a simple method for isolating and preserving aspergillus oryzae. Background technique [0002] Rice false smut (Ricefalsesmut) is caused by Aspergillus oryzae ( Villosiclavavirens (Nakata) E.Tanaka&C.Tanaka) is an important fungal disease caused by the fungus infecting the ear and forming a rice bent ball several times larger than the grain, and its surface is covered with a large number of powdery yellow or dark green chlamydospores . Rice balls not only seriously threaten the yield and quality of rice, but also produce mycotoxins that are highly toxic to humans and livestock (Koiso et al., 1994; Lietal., 1995). In recent years, with the increase in the use of N fertilizer in rice and the promotion of hybrid rice in a large area, the severity of rice false smut is increasing. In severe years, the resulting yield loss can be as high as ...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above technical problems, the present invention provides a simple method for isolating and preserving rice koji, using the method of the invention, the target strain can be quickly isolated and preserved, which solves the time-consuming and low-efficiency problem of oryza koji isolation. At the same time, it also solves the problems of pathogenicity degradation and space consumption of strains during storage