Kit based on 14 SNP loci for evaluating peripheral arterial disease prevalence risk

A peripheral arterial disease and kit technology, applied in the field of biomedicine, can solve the problems of inaccurate assessment results, susceptible to confounding factors and reverse causality, and insufficient assessment results.

Active Publication Date: 2015-12-30
CAPITALBIO CORP +1
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AI Technical Summary

Problems solved by technology

However, whether these BMI genetic loci also increase the risk of PAD, especially the risk of PAD in the Chinese population is still unclear, so it has not been included in the risk assessment system of PAD in my country
[0005] The existing methods for assessing the risk of PAD have the following defects: First, when evaluating the

Method used

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  • Kit based on 14 SNP loci for evaluating peripheral arterial disease prevalence risk
  • Kit based on 14 SNP loci for evaluating peripheral arterial disease prevalence risk
  • Kit based on 14 SNP loci for evaluating peripheral arterial disease prevalence risk

Examples

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Embodiment 1、14

[0058] Example 1. Association analysis between single nucleotide polymorphisms of 14 SNP sites and risk of peripheral arterial disease

[0059] 1. Preparation of detection kits for risk assessment of BMI and peripheral arterial disease

[0060] 1. Kit composition

[0061] The detection kit for BMI and peripheral arterial disease risk assessment developed by the inventors of the present invention contains 14 SNP sites (rs17817449 site, rs6567160 site, rs10938397 site, rs6265 site, rs574367 site, rs4715210 locus, rs4776970 locus, rs11671664 locus, rs6545814 locus, rs9356744 locus, rs261967 locus, rs12597579 locus, rs652722 locus and rs11142387 locus) gene fragment PCR primer pair, and specifically detect these 14 The 14 SNP genotypes correspond to SequenomMassARRAY single-base extension primers. The details are shown in Table 1.

[0062] Table 1 PCR primer pairs and their corresponding single-base extension primers used to detect 14 SNP sites

[0063]

[0064]

[0065]...

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Abstract

The invention discloses a kit based on 14 SNP loci for evaluating the peripheral arterial disease prevalence risk and further discloses an application for detecting a following 14 SNP locus material in preparing a product for evaluating or auxiliary evaluating of the peripheral arterial disease prevalence risk of a to-be-tested person: rs17817449 locus, rs6567160 locus, rs10938397 locus, rs6265 locus, rs574367 locus, rs4715210 locus, rs4776970 locus, rs11671664 locus, rs6545814 locus, rs9356744 locus, rs261967 locus, rs12597579 locus, rs652722 locus and rs11142387 locus. According to the kit based on 14 SNP loci for evaluating the peripheral arterial disease prevalence risk, 14 BM1 genetic loci and BMI-GRS are integrated, and the PAD prevalence risk is comprehensively evaluated; the genetic loci are tested in a large scale, and the kit is suitable for Chinese people; the test kit is economical, convenient and suitable for large-scale promotion.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a kit for evaluating the risk of peripheral artery disease based on 14 SNP sites. Background technique [0002] Peripheral arterial disease (PAD) refers to all arterial atherosclerotic lesions except coronary arteries and intracranial arteries, and common lesion areas include carotid arteries, renal arteries, and lower extremity arteries. PAD affects the blood supply to the tissue around the diseased artery, causing pain and even leading to amputation in severe cases. It not only imposes a severe physical burden on patients, but also increases the risk of disability and death from cardiovascular disease. Active and effective assessment and control of major PAD risk factors such as obesity, hypertension, and smoking can not only reduce the long-term adverse cardiovascular event rate, but also improve the quality of life. [0003] Obesity is mainly assessed by BMI, which is commonly used ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 孙义民宁光周禹稀徐敏
Owner CAPITALBIO CORP
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