Application of deoxyschizandrin to preparation of medicine for treating diabetes
The technology of Schisandrin A and a diabetes drug is applied in the field of medicine, and can solve the problems of lowering blood sugar and anti-diabetic effects without any reports, and achieves the effects of lowering blood sugar level and high sensitivity of action.
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Embodiment 1
[0022] Study on the Hypoglycemic Activity of 5μM Schizandrin A Liquid on Insulin-resistant Hepatocytes
[0023] Mouse embryonic liver cells BNLCL.2 were cultured in low-glucose DMEM containing 10% fetal calf serum at 37°C and 5% CO 2 Incubate in a cell culture incubator and replace with fresh medium every other day. When the cells grow to 80-90%, discard the culture medium, add 3 mL of PBS to rinse once, add 1 mL of 0.05% trypsin-EDTA solution to digest for about 2 minutes, add three times the amount of culture liquid to stop digestion, and incubate the cell solution at 900 r / min Centrifuge for 5 minutes, discard the supernatant, add a certain amount of culture medium, blow off the cells, take 10 μL for counting, inoculate BNLCL.2 cells in a 96-well plate with 20,000 cells per well, and set a cell-free blank control well. After culturing for 24 hours, the original medium was discarded, washed once with phosphate buffered saline (PBS), replaced with low-sugar DMEM culture medi...
Embodiment 2
[0028] Study on the Hypoglycemic Activity of 10μM Schizandrin A Liquid on Insulin-resistant Hepatocytes
[0029] Mouse embryonic liver cells BNLCL.2 were cultured in low-glucose DMEM containing 10% fetal calf serum at 37°C and 5% CO 2 Incubate in a cell culture incubator and replace with fresh medium every other day. When the cells grow to 80-90%, discard the culture medium, add 3 mL of PBS to rinse once, add 1 mL of 0.05% trypsin-EDTA solution to digest for about 2 minutes, add three times the amount of culture liquid to stop digestion, and incubate the cell solution at 900 r / min Centrifuge for 5 minutes, discard the supernatant, add a certain amount of culture medium, blow off the cells, take 10 μL for counting, inoculate BNLCL.2 cells in a 96-well plate with 20,000 cells per well, and set a cell-free blank control well. After culturing for 24 hours, the original medium was discarded, washed once with phosphate buffered saline (PBS), replaced with low-sugar DMEM culture med...
Embodiment 3
[0034] Study on the Hypoglycemic Activity of 20μM Schizandrin A Liquid on Insulin-resistant Hepatocytes
[0035] Mouse embryonic liver cells BNLCL.2 were cultured in low-glucose DMEM containing 10% fetal calf serum at 37°C and 5% CO 2 Incubate in a cell culture incubator and replace with fresh medium every other day. When the cells grow to 80-90%, discard the culture medium, add 3 mL of PBS to rinse once, add 1 mL of 0.05% trypsin-EDTA solution to digest for about 2 minutes, add three times the amount of culture liquid to stop digestion, and incubate the cell solution at 900 r / min Centrifuge for 5 minutes, discard the supernatant, add a certain amount of culture medium, blow off the cells, take 10 μL for counting, inoculate BNLCL.2 cells in a 96-well plate with 20,000 cells per well, and set a cell-free blank control well. After culturing for 24 hours, the original medium was discarded, washed once with phosphate buffered saline (PBS), replaced with low-sugar DMEM culture med...
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