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Application of embryonic fyn-related substrate efs in the prevention and treatment of enterovirus 71 infection

A related substrate, enterovirus technology, applied in the field of biomedicine

Active Publication Date: 2018-11-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0008] At present, there is no research report on the role of FYN and EFS molecules in EV71 infection of HBMEC. In-depth research on FYN and EFS molecules can not only improve the understanding of EV71 infection and pathogenic mechanism, but also provide new information for the prevention and treatment of EV71 infection. ideas and goals

Method used

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  • Application of embryonic fyn-related substrate efs in the prevention and treatment of enterovirus 71 infection
  • Application of embryonic fyn-related substrate efs in the prevention and treatment of enterovirus 71 infection
  • Application of embryonic fyn-related substrate efs in the prevention and treatment of enterovirus 71 infection

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Embodiment 1

[0057] 1 Design and synthesis of specific siRNA sequences for each host cell molecule.

[0058] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis of each target gene, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5' end of the aitisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three optimal kinetic parameter targets were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each gene, as shown...

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Abstract

The invention relates to the technical field of biomedicine, and provides a new target spot for resisting enterovirus 71 type infection and application. Human brain microvescular endothelia cells (HBMEC) serve as target cells, expression of target cell host protein is reduced by an RNA (ribonucleic acid) jamming technology so as to look for host factors capable of effectively inhibiting EV71 infected human brain microvescular endothelia cells (HBMEC), a function of protecting blood brain barrier is fulfilled, and the circumstance that viruses penetrate in the blood brain barrier and infect a central nervous system is prevented. Experiments show that embryo FYN related substrate EFS (Embryonal FYN-associated substrate) molecules play an important role in the EV71 infected HBMEC, and infection of EV71 can be inhibited obviously by reduction of expression of EFS. The invention provides application of EFS in preparation of drugs for preventing or treating enterovirus 71 type infection.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a new target and application of anti-enterovirus 71 infection. Background technique [0002] Enterovirus 71 (EV71) belongs to the Picornaviridae Enterovirus genus Human Enterovirus A, and is one of the main pathogens that cause Hand-foot-mouth disease (HFMD). Currently, hand, foot and mouth disease is breaking out and spreading in many parts of the world, especially in the Asia-Pacific region. In my country, since the outbreak of hand, foot and mouth disease in several major provinces and cities in 2008, the number of people infected and the death rate of the disease have remained high, with more than 1 million cases and nearly 1,000 deaths reported every year. The main patients with HFMD are infants and young children under 5 years old. The clinical manifestations are fever, herpes and other symptoms on the hands, feet, buttocks and oral mucosa; a small number of children c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/7088A61P31/14C12Q1/6883
Inventor 朱勇喆戚中田徐庆强赵平陈生林
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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