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A cryogenic platform for in vitro electrophysiological recording

A low-temperature, platform technology, applied in the field of in vitro electrophysiological recording, to achieve the effect of efficient utilization, simple assembly, and saving experimental time

Active Publication Date: 2017-06-16
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on this low-temperature platform

Method used

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  • A cryogenic platform for in vitro electrophysiological recording
  • A cryogenic platform for in vitro electrophysiological recording
  • A cryogenic platform for in vitro electrophysiological recording

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Please refer to figure 1 , figure 1It is a structural schematic diagram of a cryogenic platform for in vitro electrophysiological recording of the present invention. A low-temperature platform for in vitro electrophysiological recording, the low-temperature platform includes a perfusion card slot 1, a silicone hose 16, an aluminum tube 15, a peristaltic pump 7, a temperature control box 8, a magnetic table base 6, a low-temperature fixed platform 5, The cooling aluminum plate 2; the perfusion slot 1 is located on the cooling aluminum plate 2; the cooling aluminum plate 2 is fixed on the low-temperature fixing platform 5 through the fixing clip 3, and there are four fixing clips 3; There is an ice tank body 4, a fixed adjustment knob 64, a magnetic table seat 6, and an ice bag is placed in the ice tank body 4; the perfusion fluid outlet 13 is provided on the perfusion card tank 1, and the perfusion fluid outlet 13 is connected with a silicone hose 16. The other end of ...

Embodiment 2

[0059] Please refer to figure 2 , figure 2 It is a structural schematic diagram of another cryogenic platform for in vitro electrophysiological recording of the present invention. A low-temperature platform for in vitro electrophysiological recording, the low-temperature platform includes a perfusion card slot 1, a silicone hose 16, an aluminum tube 15, a peristaltic pump 7, a temperature control box 8, a magnetic table base 6, a low-temperature fixed platform 5, The cooling aluminum plate 2; the perfusion slot 1 is located on the cooling aluminum plate 2; the cooling aluminum plate 2 is fixed on the low-temperature fixing platform 5 through the fixing clip 3, and there are four fixing clips 3; There is an ice tank body 4, a fixed adjustment knob 64, a magnetic table seat 6, and an ice bag is placed in the ice tank body 4; the perfusion fluid outlet 13 is provided on the perfusion card tank 1, and the perfusion fluid outlet 13 is connected with a silicone hose 16. The othe...

Embodiment 3

[0067] Please refer to Figure 7 , Figure 7 It is a structural schematic diagram of another cryogenic platform for in vitro electrophysiological recording of the present invention. A low-temperature platform for in vitro electrophysiological recording, the low-temperature platform includes a perfusion card slot 1, a silicone hose 16, an aluminum tube 15, a peristaltic pump 7, a temperature control box 8, a magnetic table base 6, a low-temperature fixed platform 5, The cooling aluminum plate 2; the perfusion slot 1 is located on the cooling aluminum plate 2; the cooling aluminum plate 2 is fixed on the low-temperature fixing platform 5 through the fixing clip 3, and there are four fixing clips 3; There is an ice tank body 4, a fixed adjustment knob 64, a magnetic table seat 6, and an ice bag is placed in the ice tank body 4; the perfusion fluid outlet 13 is provided on the perfusion card tank 1, and the perfusion fluid outlet 13 is connected with a silicone hose 16. The othe...

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Abstract

The invention relates to a low-temperature platform for in vitro electrophysiological recording. The low-temperature platform includes a perfusion slot, a silicone hose, an aluminum tube, a peristaltic pump, a temperature control box, a magnetic table base, a low-temperature fixed platform, and a refrigeration aluminum plate; The perfusion card slot is located on the refrigeration aluminum plate; the refrigeration aluminum plate is fixed on the low-temperature fixed platform through a fixing clip, and the low-temperature solid platform is provided with an ice tank body, a fixed adjustment knob, and a magnetic table seat; the perfusion There is a perfusate outlet on the card slot, and a silicone hose is connected to the outlet of the perfusate fluid, and the other end of the silicone hose is placed in the liquid carrier; two aluminum tube interfaces are provided on the side of the perfusion card slot, and one of the aluminum tubes is The interface is connected to the peristaltic pump through the aluminum tube, and the peristaltic pump is connected to the refrigeration aluminum ring through the aluminum tube, and the other aluminum tube interface is connected to the refrigeration aluminum ring through the aluminum tube; its advantages are: temperature control, high heat dissipation efficiency, Strong anti-interference ability, convenient installation, simple operation, controllable perfusion speed, and low cost.

Description

technical field [0001] The invention relates to the technical field of in vitro electrophysiological recording, in particular to a low-temperature platform for in vitro electrophysiological recording. Background technique [0002] During in vitro electrophysiological experiments, the anesthesia of experimental animals often fails suddenly, leading to the termination of the experiment. At the same time, due to the intricate nerves in the body, when the experimental animals are anesthetized or treated with drugs, cross-effects often occur, resulting in a decrease in the accuracy and precision of the experimental results. Secondly, the operation steps in the gymnastics are cumbersome and difficult to master. Therefore, many electrophysiological experiments are often carried out in vitro. In order to reduce the metabolism of the tissue and ensure that the tissue remains active for a long time in the in vitro state, it is usually necessary to control the temperature at a low tem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L9/02G01K13/00F25D3/08
CPCF25D3/08G01K13/00B01L9/02
Inventor 兰兆辉吕为群秦逸凡许金玲魏泓毓
Owner SHANGHAI OCEAN UNIV
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