Method used for fermentation enzyme production of Serratia marcescens lipase and improving enzyme activity of Serratia marcescens lipase

Abstract

The invention discloses a method used for fermentation enzyme production of Serratia marcescens lipase and improving the enzyme activity of the Serratia marcescens lipase. A derivant is added in a fed batch manner in the fermentation process to greatly improve the output of lipase derived from Serratia marcescens, and a multiple-metal ion combination technology is adopted to activate the lipase without changing the enzyme amount in order to improve the enzyme activity of the lipase, so low-cost and high-enzyme activity is provided for production application. The method promotes application of the lipase derived from Serratia marcescens strains in production, greatly reduces the production cost, and makes fermentation enzyme production and enzyme activity improvement be simple and efficient.

Description

technical field [0001] The invention relates to an enzyme produced by Serratia marcescens lipase fermentation and a method for improving enzyme activity, belonging to the technical field of bioengineering. Background technique [0002] In 1823 Bizio discovered Serratia marcescens ( Serratiamarcesensens ) belongs to the Serratia genus under the Enterobacteriaceae family, and can secrete a variety of extracellular enzymes such as lipase, chitinase, metalloprotease, thiol protease, etc. At present, the secretion mechanism of Serratia marcescens lipase has been studied clearly, which is the ABC-transpoter mechanism of Gram-negative bacteria. Due to the great application potential, the lipase genes of many strains have been cloned and expressed. It is worth mentioning that the lipase of Serratia marcescens Sr418000 was first cloned and expressed by Japanese scientists, and the enzyme was immobilized in the membrane reactor. The above is used to resolve the racemate trans-4-meth...

AI Technical Summary

Problems solved by technology

However, due to the problem that the current Serratia marcescens lipase has insufficient enzyme activity or low expression l