Genetic engineering bacterium for producing succinic acid, and construction and application thereof

A technology of genetically engineering bacteria to produce succinic acid, applied in the field of bioengineering, can solve the problems of inability to utilize glucose, growth inhibition, etc., and achieve the effects of simple and feasible fermentation method, strong acid production capacity, and reduced production costs
CN102864116BActive Publication Date: 2014-03-05NANJING TECH UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING TECH UNIV
Publication Date
2014-03-05

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Abstract

The invention provides a genetic engineering bacterium strain for producing succinic acid. The genetic engineering bacterium strain is named as Escherichia coli BA016, the preserving number registration number CCTCC NO is M 2012350. The invention further provides a construction method of the strain and a method for producing succinic acid by fermentation, recombinant escherichia coli can grow by glucose metabolism through joint excessive expression of exogenous pyruvic carboxylase and nicotinic acid ribose phosphate transferase, generation of by-product pyruvic acid is reduced, and accordingly the yield and production intensity of succinic acid are greatly improved.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and relates to a succinic acid-producing genetically engineered bacterium and its construction and application, in particular to a genetically engineered bacterium that efficiently utilizes glucose to grow and produce succinic acid Escherichia coli Construction of BA016 and method for its production of succinic acid. Background technique

[0002] Succinic acid, commonly known as succinic acid, as a common natural organic acid, widely exists in animals, plants and microorganisms, and many anaerobic microorganisms produce succinic acid as the main end product of their energy metabolism. As an excellent C4 platform compound, succinic acid can be widely used in pharmaceuticals, fine chemical products and biodegradable polymers. The report of the US Department of Energy listed succinic acid as the 12 most potential in the future. No. 1 among bio-based bulk chemicals.. Many anaerobic microorg...

Claims

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