Cpg-oligodeoxynucleotide, immunogenic composition comprising the same, and methods for preparing the composition and stimulating immune response thereby

A technology of oligodeoxynucleotide and immune composition, applied in biochemical equipment and methods, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of low activity and achieve the effect of less side effects

Active Publication Date: 2016-04-27
台湾卫生研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Existing studies have shown that compared with human (h) TLR9 and mouse (m) TLR9, rabbit (rab) TLR9 has a wider ligand recognition profile (ligandrecognition profile) for CpG-ODN with GTCGTT motif or GACGTT motif; However, CpG-ODNs currently developed to activate human or mouse cells have low activity against rabTLR9(14)

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  • Cpg-oligodeoxynucleotide, immunogenic composition comprising the same, and methods for preparing the composition and stimulating immune response thereby
  • Cpg-oligodeoxynucleotide, immunogenic composition comprising the same, and methods for preparing the composition and stimulating immune response thereby
  • Cpg-oligodeoxynucleotide, immunogenic composition comprising the same, and methods for preparing the composition and stimulating immune response thereby

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Embodiment Construction

[0059] The technical content of the present invention will become apparent through the detailed description of the following embodiments and the illustrations of the related drawings.

[0060] definition

[0061] "About" or "approximately", when used in connection with a measurable numerical variable, refers to the indicated value of the variable and is within the experimental error of the indicated value (for example, a 95% confidence interval for the mean) or within the indicated value. All values ​​of the variable within 10% of , from which the maximum value is taken.

[0062] "Adjuvant" refers to any substance that enhances the humoral or cellular immune response to an antigen. Adjuvants are generally used for two purposes: to delay the release of antigens from the injection site, and to stimulate the immune system.

[0063] "Antibody" refers to an immunoglobulin molecule capable of binding a specific antigen as a result of an immune response to that antigen. Immunoglob...

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Abstract

CpG-oligodeoxynucleotides (CpG-ODN), as potent immune stimuli developed for using as adjuvant in different species, are disclosed herein. TLR9 is the cellular receptor for CpG-ODN, and current developed CpG-ODN has low activity to rabbit TLR9. Here, a type of CpG-ODN containing a GACGTT or AACGTT motif in about 11-14 deoxynucleotides was demonstrated to have potent immunostimulatory activity to rabbit TLR9, and capable of boosting a less toxic and potent antibody response in rabbits.

Description

technical field [0001] Cross-references to related applications [0002] This application claims the benefit of US Provisional Application No. 61 / 856,268, filed July 19, 2013, the disclosure of which is hereby incorporated by reference in its entirety. [0003] The present invention relates generally to CpG oligodeoxynucleotides, immune compositions comprising CpG oligodeoxynucleotides, methods for preparing immune compositions and methods for stimulating immune responses, in particular CpG comprising a GACGTT or AACGTT motif Oligodeoxynucleotides, immune compositions comprising CpG oligodeoxynucleotides, methods for preparing the immune compositions, and methods for stimulating TLR9 to activate an immune response. Background technique [0004] CpG oligodeoxynucleotides (CpG-ODNs) are potent immune stimulators, and Toll-like receptor 9 (TLR9) is its cellular receptor (1-5). Several immune effects may result from TLR9 activation by CpG-ODN, including increased T helper (Th)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39
CPCA61K39/39A61K2039/55561A61P37/04A61K2039/55505C12N15/117C12N2310/17
Inventor 庄宗显赖朝阳
Owner 台湾卫生研究院
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